LncRNA-000133 from secondary hair follicle of Cashmere goat: identification, regulatory network and its effects on inductive property of dermal papilla cells
LncRNA-000133 from secondary hair follicle of Cashmere goat: identification, regulatory network and its effects on inductive property of dermal papilla cells
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绒山羊次生毛囊LncRNA-000133：鉴定、调控网络及其对真皮乳头细胞诱导特性的影响
DOI：
10.1080/10495398.2018.1553788
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发表时间：
2020
影响因子：
3.7
通讯作者：
Bai Wenlin
中科院分区：
文献类型：
--
作者：
Zheng Yuanyuan;Wang Zeying;Zhu Yubo;Wang Wei;Bai Man;Jiao Qian;Wang Yanru;Zhao Sujun;Yin Xianbo;Guo Dan;Bai Wenlin
长非编码RNA(LncRNAs)是一类长度为200 的非蛋白结合RNAs，被认为在蛋白质编码基因的表达调控中起着关键作用。在这里，我们从绒山羊次级毛囊中鉴定并鉴定了一个新的cRNA-000133，并通过过表达分析评价了其对毛乳头细胞诱导特性的潜在作用。表达分析表明，在绒山羊绒毛发育过程中，lncRNA-000133在生长期的表达水平显著高于在胎盘组织中的表达水平，提示该基因可能参与了绒毛纤维形成和生长过程中绒毛发育过程中绒毛再生的过程。结合甲基化分析，我们认为LncRNA-000133基因5‘调控区的甲基化可能与其在绒山羊体表皮肤病中的表达抑制有关。CERNA调控网络表明，lncRNA-000133和相关miRNA与其靶基因之间存在丰富而复杂的调控关系。IncRNA-000133的过表达导致毛乳头细胞ET-1、SCF、碱性磷酸酶和LEF1的相对表达显著增加，提示IncRNA-000133可能参与了毛乳头细胞的诱导特性。
Long noncoding RNAs (lncRNAs), a class of non-protein conding RNAs > 200 nt in length, were thought to play critical roles in regulating the expression of protein-coding genes. Here, we identified and characterized a novel lncRNA-000133 from the secondary hair follicle (SHF) of cashmere goat with its ceRNA network analysis, as well as, its potential effects on inductive property of dermal papilla cells were evaluated through overexpression analysis. Expression analysis indicated that lncRNA-000133 had a significantly higher expression at anagen than that at telogen in SHF of Cashmere goat, suggesting that lncRNA-000133 might be involved in the reconstruction of SHF with the formation and growth of cashmere fiber. Taken together with methylation analysis, we showed that 5’ regulatory region methylation of the lncRNA-000133 gene might be involved in its expression suppression in SHF of Cashmere goat. The ceRNA regulatory network showed that a rich and complex regulatory relationship between lncRNA-000133 and related miRNAs with their target genes. The overexpression of lncRNA-000133 led to a significant increasing in the relative expression of ET-1, SCF, ALP and LEF1 in dermal papilla cells suggesting that lncRNA-000133 appears to contribute the inductive property of dermal papilla cells.