Interleukin-4-induced STAT6 recognizes and activates a target site in the promoter of the interleukin-4 receptor gene
Interleukin-4-induced STAT6 recognizes and activates a target site in the promoter of the interleukin-4 receptor gene
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DOI：
10.1074/jbc.271.41.25555
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发表时间：
1996-10-11
影响因子：
4.8
通讯作者：
Reich, NC
中科院分区：
文献类型：
--
作者：
Kotanides, H;Reich, NC
本研究探讨STAT6在白介素4刺激的基因表达中的作用。在白介素4受体基因的启动子中发现了一个特异的STAT6 DNA结合靶点，并且STAT6被证明通过这个位点参与了基因表达的激活。STAT6可以介导含有白介素4受体STAT6结合位点的异源报告基因的转录。此外，有证据表明，STAT6DNA结合特异性对转录调控有明显的影响，因为转录不是从一个可胜任的但不同的DNA结合部位刺激的。为了证实STAT6在基因激活中的作用，我们制备了STAT6突变蛋白，并分析了它们在白介素4诱导的转录中的功能。尽管白细胞介素2伽马链受体亚单位已被证明是白细胞介素4受体的一个组成部分，但它不是特定基因激活所必需的。
This study addresses the function of STAT6 in interleukin-4-stimulated gene expression. A specific STAT6 DNA-binding target site has been identified in the promoter of the interleukin-4 receptor gene, and STAT6 is shown to be involved in mediating activation of gene expression via this site. STAT6 can mediate transcription of a heterologous reporter gene construct containing the interleukin-4 receptor STAT6 binding site. In addition, evidence is provided that demonstrates a distinct effect of STAT6 DNA binding specificity on transcriptional regulation since transcription was not stimulated from a competent but different DNA binding site. To confirm the role of STAT6 in gene activation, STAT6 mutant proteins were generated and analyzed for their ability to function in interleukin-4-induced transcription. Although the interleukin-2 gamma chain receptor subunit has been demonstrated to be a component of the interleukin-4 receptor, it is not required for specific gene activation.