Spatially resolved analysis of small molecules by matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI)
Spatially resolved analysis of small molecules by matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI)
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DOI：
10.1111/j.1469-8137.2011.03970.x
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发表时间：
2012-02-01
期刊：
影响因子：
9.4
通讯作者：
Mock, Hans-Peter
中科院分区：
文献类型：
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作者：
Peukert, Manuela;Matros, Andrea;Mock, Hans-Peter
基质辅助激光解吸/电离质谱学成像(MALDI-MSI)为分析小分子和蛋白质在组织中的空间分布提供了手段。这种成像技术在医学和药学研究中很常见，但在植物科学中的应用却是最近的事。更广泛的引进需要对植物组织进行特定的适应。为了获得高质量的光谱，为化合物提供足够的空间分辨率，样品制备是至关重要的。切片、基质的选择和基质沉积的方法都需要优化。在这里，我们介绍了我们目前在冷冻解剖的未成熟大麦(Hordeum Usgare)籽粒和烟草(Nictiana Tabacum)根中检测小分子的方法。提供了MALDI-MSI测量的例子，并讨论了生物复制的重复性水平。最后，我们讨论了MALDI-MSI如何为应用植物研究做出贡献。
Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) of tissues provides the means to analyse the spatial distributions of small molecules and proteins within tissues. This imaging technique is commonplace in medicinal and pharmaceutical research, but its application in plant science is very recent. Broader introduction requires specific adaptations for plant tissues. Sample preparation is of paramount importance in order to obtain high-quality spectra providing sufficient spatial resolution for compounds. Optimization is required for sectioning, choice of matrix and means of matrix deposition.Here, we present our current protocols for the detection of small molecules in cryodissected immature barley (Hordeum vulgare) grains and tobacco (Nicotiana tabacum) roots.Examples of MALDI-MSI measurements are provided, and the level of reproducibility across biological replicates is addressed. Furthermore, our approaches for the validation of distribution patterns and for the identification of molecules are described.Finally, we discuss how MALDI-MSI can contribute to applied plant research.