Biomimetic organization:: Octapeptide self-assembly into nanotubes of viral capsid-like dimension
Biomimetic organization:: Octapeptide self-assembly into nanotubes of viral capsid-like dimension
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DOI：
10.1073/pnas.1730609100
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发表时间：
2003-09-02
影响因子：
11.1
通讯作者：
Artzner, F
中科院分区：
文献类型：
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作者：
Valéry, C;Paternostre, M;Artzner, F
复杂分子的可控自组装成为定义良好的层次化结构是制备纳米结构的一条很有前途的途径。这些纳米结构可以通过自然存在的蛋白质实现，如烟草花叶病毒、衣壳蛋白、微管蛋白、肌动蛋白等。在这里，我们报告了一种简单的替代方法，该方法基于合成的治疗性八肽兰瑞肽在水中形成的自组装纳米管。我们使用了多学科的方法，包括光学和电子显微镜，振动光谱，以及小角和广角X射线散射，以阐明该系统所展示的结构层次。结果表明，纳米管呈六角形堆积，在管径(244埃)和壁厚(约18埃)上具有高度的单分散性。此外，通过对分子结构进行适当的修饰，直径是可调的。纳米管的自组装是通过两亲性驱动的β-片层的缔合和系统的芳香族/脂肪族侧链分离来进行的。这个原始而简单的系统是研究从头分子或淀粉样肽产生的复杂自组装过程的独特例子。
The controlled self-assembly of complex molecules into well defined hierarchical structures is a promising route for fabricating nanostructures. These nanoscale structures can be realized by naturally occurring proteins such as tobacco mosaic virus, capsid proteins, tubulin, actin, etc. Here, we report a simple alternative method based on self-assembling nanotubes formed by a synthetic therapeutic octapeptide, Lanreotide in water. We used a multidisciplinary approach involving optical and electron microscopies, vibrational spectroscopies, and small and wide angle x-ray scattering to elucidate the hierarchy of structures exhibited by this system. The results revealed the hexagonal packing of nanotubes, and high degree of monodispersity in the tube diameter (244 Angstrom) and wall thickness (approximate to18 Angstrom). Moreover, the diameter is tunable by suitable modifications in the molecular structure. The self-assembly of the nanotubes occurs through the association of beta-sheets driven by amphiphilicity and a systematic aromatic/aliphatic side chain segregation. This original and simple system is a unique example for the study of complex self-assembling processes generated by de novo molecules or amyloid peptides.