Blue-Emissive Upconversion Nanoparticles for Low-Power-Excited Bioimaging in Vivo
Blue-Emissive Upconversion Nanoparticles for Low-Power-Excited Bioimaging in Vivo
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DOI：
10.1021/ja3003638
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发表时间：
2012-03-21
影响因子：
15
通讯作者：
Li, Fuyou
中科院分区：
文献类型：
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作者：
Liu, Qian;Yang, Tianshe;Li, Fuyou
通过在二氧化硅纳米粒子中共负载敏化剂（八乙基卟啉钯配合物）和消灭剂（9,10-二苯基蒽），成功制备了基于三重态-三重态湮没（TTA）的水溶性上转换发光（UCL）纳米粒子。纳米粒子在水溶液中的上转换发光量子产率可高达4.5%。通过连续动力学扫描测定，纳米粒子具有优异的光稳定性。这种基于 TTA 的上转换纳米颗粒显示出低细胞毒性，并成功用于标记具有非常高信噪比的活细胞。使用纳米粒子作为探针的 UCL 成像能够完全消除来自生物样品的内源荧光团或共标记荧光探针的背景荧光。特别是，这种蓝色发射上转换纳米颗粒在连续波532激光（8.5 mW cm(-2)）低功率密度激发下成功应用于活体小鼠体内淋巴结成像，具有优异的信噪比（> 25）。这种以蓝色发射上转换纳米粒子作为探针的高对比度和低功率体内激发生物成像可以扩展目前可用的体外和体内发光生物成像的库。
Water-soluble upconversion luminescent (UCL) nanoparticles based on triplet-triplet annihilation (TTA) were successfully prepared by coloading sensitizer (octaethylporphyrin Pd complex) and annihilator (9,10-diphenylanthracene) into silica nanoparticles. The upconversion luminescence quantum yield of the nanoparticles can be as high as 4.5% in aqueous solution. As determined by continuous kinetic scan, the nanopartides have excellent photostability. Such TTA-based upconversion nanopartides show low cytotoxicity and were successfully used to label living cells with very high signal-to-noise ratio. UCL imaging with the nanoparticles as probe is capable of completely eliminating background fluorescence from either endogenous fluorophores of biological sample or the colabeled fluorescent probe. In particular, such blue-emissive upconversion nanoparticles were successfully applied in lymph node imaging in vivo of living mouse with excellent signal-to-noise ratio (> 25), upon low-power density excitation of continuous-wave 532 laser (8.5 mW cm(-2)). Such high-contrast and low-power excited bioimaging in vivo with a blue-emissive upconversion nanopartide as probe may extend the arsenal of currently available luminescent bioimaging in vitro and in vivo.