A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat
A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat
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DOI：
10.1534/genetics.104.034868
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发表时间：
2004-10-01
期刊：
影响因子：
3.3
通讯作者：
Gill, BS
中科院分区：
文献类型：
--
作者：
Qi, LL;Echalier, B;Gill, BS
由于普通小麦（Triticum aestivum L.，2n = 6x = 42，AABBDD）基因组大小高达 17,300 Mb，因此对表达部分进行测序和作图是基因发现的合理第一步。在这里，我们报告了使用一组小麦非整倍体和缺失库，通过 Southern 杂交将 7104 个表达序列标签 (EST) 单基因映射到染色体箱图谱中。每个 EST 平均检测到 4.8 个限制性片段和 2.8 个位点。 B 基因组 (5774) 中定位的位点比 A (5173) 或 D (5146) 基因组中的位点更多。 D 基因组的 EST 密度显着高于 A 或 B 基因组。一般来说，EST 密度相对于着丝粒的物理距离而增加。大多数 EST 密集区域位于染色体的远端部分。大多数农艺学上重要的基因位于 EST 密集区域。 EST 的染色体箱图是用于 SNP 分析、比较作图、结构和功能分析以及多倍体进化的独特资源，并为构建小麦基因组的序列就绪 BAC 重叠群图谱提供了框架。
Because of the huge size of the common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) genome of 17,300 Mb, sequencing and mapping of the expressed portion is a logical first step for gene discovery. Here we report mapping of 7104 expressed sequence tag (EST) unigenes by Southern hybridization into a chromosome bin map using a set of wheat aneuploids and deletion stocks. Each EST detected a mean of 4.8 restriction fragments and 2.8 loci. More loci were mapped in the B genome (5774) than in the A (5173) or D (5146) genomes. The EST density was significantly higher for the D genome than for the A or B. In general, EST density increased relative to the physical distance from the centromere. The majority of EST-dense regions are in the distal parts of chromosomes. Most of the agronomically important genes are located in EST-dense regions. The chromosome bin map of ESTs is a unique resource for SNP analysis, comparative mapping, structural and functional analysis, and polyploid evolution, as well as providing a framework for constructing a sequence-ready, BAC-contig map of the wheat genome.