The characterization of novel monomeric creatine kinases in the early branching Alveolata species, Perkinsus marinus: Implications for phosphagen kinase evolution
The characterization of novel monomeric creatine kinases in the early branching Alveolata species, Perkinsus marinus: Implications for phosphagen kinase evolution
复制标题
DOI：
10.1016/j.cbpb.2022.110758
复制
发表时间：
2022-05-26
影响因子：
2.2
通讯作者：
Suzuki, Tomohiko
中科院分区：
文献类型：
--
作者：
Fraga, Dean;Ellington, W. Ross;Suzuki, Tomohiko
单细胞软体动物寄生虫Perkinsus marinus的基因组包含至少五个编码推定肌酸激酶（CK）的基因，肌酸激酶是一种磷酰基转移酶，在细胞能量交易中发挥关键作用。三个的表达和动力学分析表明，他们是真正的CK的催化性能的范围内的典型后生动物CK。将P. marinus CK与该家族中的一系列CK二聚体和其他二聚体磷酰基转移酶（磷酸原激酶）的序列比较表明，P. marinus CK缺乏参与二聚体稳定的一些关键残基，这是所有先前表征的CK共有的特征。所有三种表达的海泛藻CK构建体的尺寸排阻色谱表明它们是单体的，与观察到的一些关键的二聚体稳定残基的缺乏一致。对P. marinus CK和具有广泛的单体和二聚体磷酸原激酶的推定甲藻CK的系统发育分析表明，帕金虫CK与甲藻CK形成了一个独特的、得到充分支持的进化枝，甲藻CK也缺乏二聚体稳定残基。对海原甲藻基因组数据的分析表明，存在与肌酸生物合成相关的两种酶的推定基因。CK在高等生物中在显示高和可变ATP周转率的细胞类型中的能量缓冲中起关键作用。多种肌酸激酶和肌酸生物合成途径的存在表明，这种单细胞寄生虫具有CK介导的能量缓冲的完整分子机制。
The genome of the unicellular molluscan parasite Perkinsus marinus contains at least five genes coding for putative creatine kinases (CK), a phosphoryl transfer enzyme which plays a key role in cellular energy transactions. Expression and kinetic analyses of three of the P. marinus CKs revealed them to be true CKs with catalytic properties in the range of typical metazoan CKs. A sequence comparison of the P. marinus CKs with a range of CK dimers and other dimeric phosphoryl transfer enzymes in this family (phosphagen kinases) showed that the P. marinus CKs lacked some of the critical residues involved in dimer stabilization, a trait all previously characterized CKs share. Size exclusion chromatography of all three expressed P. marinus CK constructs indicated they are monomeric, consistent with the observed lack of some critical dimer stabilizing residues. Phylogenetic analyses of the P. marinus CKs and putative dinoflagellate CKs with a broad range of monomeric and dimeric phosphagen kinases revealed that the Perkinsus CKs form a distinct, well-supported clade with dinoflagellate CKs which also lack the dimer stabilizing residues. Analysis of the genomic data for P. marinus showed the presence of putative genes for the two enzymes associated with creatine biosynthesis. CK in higher organisms plays a critical role in energy buffering in cell types displaying high and variable rates of ATP turnover. The presence of multiple CKs and the creatine biosynthetic pathway in P. marinus indicates that this unicellular parasite has the full complement of molecular machinery for CK-mediated energy buffering.