Neuromedin U receptor-2 mRNA and HCN channels mRNA expression in NMU-sensitive neurons in rat hypothalamic paraventricular nucleus
Neuromedin U receptor-2 mRNA and HCN channels mRNA expression in NMU-sensitive neurons in rat hypothalamic paraventricular nucleus
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DOI：
10.1016/j.neulet.2004.10.033
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发表时间：
2005-02-01
影响因子：
2.5
通讯作者：
Kannan, H
中科院分区：
文献类型：
--
作者：
Qiu, DL;Chu, CP;Kannan, H
我们的特点是神经介肽U（NMU）敏感的神经元在大鼠室旁核（PVN）使用全细胞膜片钳记录和单细胞逆转录-多重聚合酶链反应（单细胞RT-mPCR）。完成全细胞记录后，使用单细胞RT-mPCR检测NMDA敏感神经元的催产素（OT）、加压素（VP）和促肾上腺皮质激素释放激素（CRH）mRNA表达。在23个NMU敏感神经元中，82%表达OT mRNA，9%表达VP mRNA，9%不表达特异性表型mRNA。此外，NMU敏感神经元（23/23）主要表达NMU受体2（NMUR-2）mRNA，共表达HCN 1通道mRNA、HCN 2通道mRNA和HCN 3通道mRNA，但不表达HCN 4通道mRNA。这些结果表明，NMU调节PVN推定的小细胞神经元的功能，并参与通过NMU受体2增强HCN离子通道活性来调节OTergic和VPergic神经元。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
We have characterized the neuromedin U (NMU)-sensitive neurons in the rat paraventricular nucleus (PVN) using whole-cell patch-clamp recordings and single-cell reverse transcription-multiplex polymerase chain reaction (single-cell RT-mPCR). Following completion of whole-cell recording, the NMU-sensitive neurons were examined for oxytocin (OT), vasopressin (VP), and corticotrophin-releasing hormone (CRH) mRNA expression using single-cell RT-mPCR. Of the NMU-sensitive neurons (n = 23), 82% expressed OT mRNA, 9% expressed VP mRNA, 9% did not express the detected specific phenotypes mRNA. Further, the NMU-sensitive neurons (23/23) predominantly expressed NMUreceptor 2 (NMUR-2) mRNA, co-expressed HCN1 channel mRNA, HCN2 channel mRNA, and HCN3 channel mRNA but not HCN4 channel mRNA. These results suggest that NMU modulates the function of the PVN putative parvocellular neurons and is involved in the regulation of OTergic and VPergic neurons by enhanced HCN ion channels activity via NMU-receptor 2. (C) 2004 Elsevier Ireland Ltd. All rights reserved.