CHARACTERIZATION AND TRANSCRIPTIONAL PROFILES OF A DROSOPHILA GENE ENCODING AN INSECT DEFENSIN - A STUDY IN INSECT IMMUNITY
CHARACTERIZATION AND TRANSCRIPTIONAL PROFILES OF A DROSOPHILA GENE ENCODING AN INSECT DEFENSIN - A STUDY IN INSECT IMMUNITY
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DOI：
10.1111/j.1432-1033.1994.tb18730.x
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发表时间：
1994-04-01
期刊：
影响因子：
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通讯作者：
HOFFMANN, JA
中科院分区：
文献类型：
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作者：
DIMARCQ, JL;HOFFMANN, D;HOFFMANN, JA
昆虫防御素是一个4-kDa，阳离子，诱导抗菌肽家族，具有六个半胱氨酸残基，参与三个分子内二硫桥。它们得名于与哺乳动物嗜中性粒细胞和巨噬细胞的防御素的某些序列相似性。我们报道了一种来自果蝇的新型防御素亚型的特征和编码前防御素基因的克隆。该基因无内含子，存在于单拷贝/单倍体基因组中，位于第二条染色体右臂的46CD位置。对该基因上游区域的分析显示，存在多个假定的顺式调控序列，类似于哺乳动物急性期反应基因的调控基序。转录谱表明果蝇防御素基因是由急性期动力学的细菌攻击诱导的。它也在变态过程中缺乏免疫挑战时表达。这些和其他关于果蝇防御素基因的数据使我们认为昆虫和哺乳动物的防御素是独立进化的。
Insect defensins are a family of 4-kDa, cationic, inducible antibacterial peptides which bear six cysteine residues engaged in three intramolecular disulfide bridges. They owe their name to certain sequence similarities with defensins from mammalian neutrophiles and macrophages. We report the characterization of a novel defensin isoform from Drosophila and the cloning of the gene encoding a preprodefensin. The gene, which is intronless and present in a single copy/haploid genome, maps at position 46CD on the right arm of the second chromosome. The analysis of the upstream region of the gene reveals the presence of multiple putative cis-regulatory sequences similar to mammalian regulatory motifs of acute-phase-response genes. Transcriptional profiles indicate that the Drosophila defensin gene is induced by bacterial challenge with acute-phase kinetics. It is also expressed in the absence of immune challenge during metamorphosis. These and other data on the Drosophila defensin gene lead us to suggest that insect and mammalian defensins have evolved independently.