PtoMYB142, a poplar R2R3-MYB transcription factor, contributes to drought tolerance by regulating wax biosynthesis
PtoMYB142, a poplar R2R3-MYB transcription factor, contributes to drought tolerance by regulating wax biosynthesis
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PtoMYB142 是杨树 R2R3-MYB 转录因子，通过调节蜡生物合成有助于耐旱
DOI：
10.1093/treephys/tpac060
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发表时间：
2022-05-28
期刊：
影响因子：
4
通讯作者：
Luo，Keming
中科院分区：
文献类型：
--
作者：
Song，Qin;Kong，Lingfei;Luo，Keming
干旱是限制植物生长发育的主要环境因子之一。因此，植物在干旱胁迫下已经进化出防止水分损失的策略，例如气孔关闭，维持根系吸水，增强茎水分运输以及角质层蜡的合成和沉积。然而，木本植物表皮蜡生物合成调节响应干旱的分子证据有限。本研究中，我们从毛白杨（Populus tomentosa卡尔）中鉴定了一个MYB转录因子（PtoMYB 142）。绒毛草过量表达PtoMYB 142（PtoMYB 142-OE）可增加白杨叶片蜡质积累，显著增强其抗旱性。结果表明，干旱胁迫显著诱导了蜡质生物合成基因CER 4和3-酮脂酰辅酶A合成酶（KCS）的表达，并在PtoMYB 142-OE株系中显著上调。生化分析证实PtoMYB 142可直接与CER 4和KCS 6的启动子结合，调控其在P.绒毛草综上所述，这项研究表明，PtoMYB 142调节表皮蜡的生物合成，以适应缺水条件。
Drought is one of the main environmental factors that limit plant development and growth. Accordingly, plants have evolved strategies to prevent water loss under drought stress, such as stomatal closure, maintenance of root water uptake, enhancement of stem water transport, and synthesis and deposition of cuticular wax. However, the molecular evidence of cuticular wax biosynthesis regulation in response to drought is limited in woody plants. Here, we identified an MYB transcription factor,Populus tomentosa Carr.MYB transcription factor (PtoMYB142), in response to drought stress fromP. tomentosa. Over-expression ofPtoMYB142(PtoMYB142-OE) resulted in increased wax accumulation in poplar leaves, and significantly enhanced drought resistance. We found that the expression of wax biosynthesis genesCER4and 3-ketoacyl CoA synthase (KCS) were markedly induced under drought stress, and significantly up-regulated inPtoMYB142-OE lines. Biochemical analysis confirmed that PtoMYB142 could directly bind to the promoter ofCER4andKCS6, and regulate their expression inP. tomentosa. Taken together, this study reveals that PtoMYB142 regulates cuticular wax biosynthesis to adapt to water-deficient conditions.