Reniochalistatins A-E, Cyclic Peptides from the Marine Sponge Reniochalina stalagmitis
Reniochalistatins A-E, Cyclic Peptides from the Marine Sponge Reniochalina stalagmitis
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Reniochalistatins A-E，来自海洋海绵 Reniochalina 石笋的环肽。
DOI：
10.1021/np5006778
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发表时间：
2014-12-01
影响因子：
5.1
通讯作者：
Lin, Hou-Wen
中科院分区：
文献类型：
--
作者：
Zhan, Kai-Xuan;Jiao, Wei-Hua;Lin, Hou-Wen
从中国南海永兴岛附近的石笋海绵中分离和鉴定了5个新的环肽(包括4个七肽和1个八肽)，即Reniochalistatins A-E(1-5)。根据HRESIMS，一维和二维核磁共振波谱数据以及MALDI-TOF/TOF数据对它们的结构进行了指认和序列分析。用手性相高效液相色谱法和Marfey‘s分析确定了所有氨基酸残基的绝对构型。用不同的肿瘤细胞株(RPMI-8226、MGC-803、HL-60、HepG2和HeLa)进行的不同细胞毒实验表明，环状八肽Reniochalistatin E具有生物活性。
Five new cyclic peptides (including four heptapeptides and one octapeptide), reniochalistatins A-E (1-5), were isolated and characterized from the marine sponge Reniochalina stalagmitis collected off Yongxing Island in the South China Sea. Their structures were assigned on the basis of HRESIMS, 1D and 2D NMR spectroscopic data, and MALDI-TOF/TOF data for sequence analysis. The absolute configurations of all of the amino acid residues were determined using chiral-phase HPLC and Marfey's analysis. The cyclic octapeptide reniochalistatin E showed biological activity in various cytotoxicity assays employing different tumor cell lines (RPMI-8226, MGC-803, HL-60, HepG2, and HeLa).