Net-Shaped DNA Nanostructures Designed for Rapid/Sensitive Detection and Potential Inhibition of the SARS-CoV-2 Virus
Net-Shaped DNA Nanostructures Designed for Rapid/Sensitive Detection and Potential Inhibition of the SARS-CoV-2 Virus
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DOI：
10.1021/jacs.2c04835
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发表时间：
2022-07-26
影响因子：
15
通讯作者：
Wang, Xing
中科院分区：
文献类型：
--
作者：
Chauhan, Neha;Xiong, Yanyu;Wang, Xing
我们提出了一种网状 DNA 纳米结构（本文称为“DNA 网”）设计策略，通过位于 DNA 网上的适体（针对野生型刺突-RED）与病毒外表面展示的三聚体刺突糖蛋白之间的空间模式匹配和多价相互作用，选择性识别和高亲和力捕获完整的 SARS-CoV-2 病毒颗粒。 DNA Net 适体携带设计的纳米开关，在病毒结合时释放荧光信号，可以使用手持式荧光计轻松读取荧光信号，以进行快速（10 分钟内）、自然兼容且廉价（类似于 1.26 美元/测试）的 COVID-19 检测分析。 DNA Net 适体还可以将单体适体增强近 1 x 10(3) 倍，从而阻止细胞培养物中真正的野生型 SARS-CoV-2 感染。此外，我们的 DNA Net 设计原则和策略可以定制，以应对其他危及生命且具有经济影响力的病毒，如流感和 HIV，其表面携带 I 类病毒包膜糖蛋白，如三聚体形式的 SARS-CoV-2 刺突。
We present a net-shaped DNA nanostructure (called "DNA Net" herein) design strategy for selective recognition and high-affinity capture of intact SARS-CoV-2 virions through spatial pattern-matching and multivalent interactions between the aptamers (targeting wild-type spike-RED) positioned on the DNA Net and the trimeric spike glycoproteins displayed on the viral outer surface. Carrying a designer nanoswitch, the DNA Net-aptamers release fluorescence signals upon virus binding that are easily read with a handheld fluorimeter for a rapid (in 10 min), ature compatible, and inexpensive (similar to$1.26/test) COVID-19 test assay. The DNA Net-aptamers also impede authentic wild-type SARS-CoV-2 infection in cell culture with a near 1 x 10(3)-fold enhancement of the monomeric aptamer. Furthermore, our DNA Net design principle and strategy can be customized to tackle other life-threatening and economically influential viruses like influenza and HIV, whose surfaces carry class-I viral envelope glycoproteins like the SARS-CoV-2 spikes in trimeric forms.