DESTRUCTION OF MICROFILAMENT BUNDLES IN MOUSE EMBRYO FIBROBLASTS TREATED WITH INHIBITORS OF ENERGY-METABOLISM
DESTRUCTION OF MICROFILAMENT BUNDLES IN MOUSE EMBRYO FIBROBLASTS TREATED WITH INHIBITORS OF ENERGY-METABOLISM
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DOI：
10.1016/0014-4827(80)90446-2
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发表时间：
1980-01-01
影响因子：
3.7
通讯作者：
TINT, IS
中科院分区：
文献类型：
--
作者：
BERSHADSKY, AD;GELFAND, VI;TINT, IS
采用抗肌动蛋白抗体免疫荧光和电镜观察能量代谢抑制剂[叠氮化羰基氰化物-间氯苯腙钠，2,4-二硝基苯酚和寡霉素]治疗小鼠成纤维细胞时肌动蛋白的分布。抑制剂诱导含有肌动蛋白的微丝束逐渐瓦解。在该过程的第一阶段，束分解成单独的片段；后来，在抑制剂处理的细胞中只能发现小块的肌动蛋白。这种转化大约需要90分钟，并且是完全可逆的，因为细胞在无抑制剂的生长培养基中孵育后，微丝束被恢复。抑制剂不改变肌动蛋白在葡萄糖存在下的分布。它们的作用显然是由于细胞中ATP水平的降低。用抑制剂孵育90分钟不会显著改变细胞形状或微管系统。能量代谢抑制剂阻止细胞松弛素对细胞的作用。细胞松弛素B或细胞松弛素D迅速破坏微细丝束并引起细胞乔木化。在细胞松弛素和任何抑制剂的混合物中培养的细胞中，微丝束的破坏需要90分钟，并且不伴有细胞形态的剧烈变化，因此该过程与单独存在抑制剂的微丝束破坏过程难以区分。
Immunofluorescence with an antiactin antibody and EM were used to study the distribution of actin in cultured mouse fibroblasts during treatment with inhibitors of energy metabolism [sodium azide carbonylcyanide-m-chlorophenylhydrazone,2,4-dinitrophenol and oligomycin]. The inhibitors induce gradual disorganization of actin-containing microfilament bundles. At the 1st stage of the process the bundles degrade into separate fragments; later only small patches of actin can be found in the inhibitor-treated cells. This transformation takes about 90 min and is fully reversible as microfilament bundles are recovered after incubation of the cells in the inhibitor-free growth medium. The inhibitors do not alter actin distribution in the presence of glucose. Their action apparently is due to a reduction of the ATP level in the cells. A 90 min incubation with the inhibitors does not markedly alter either the cell shape or the microtubule system. Inhibitors of the energy metabolism prevent cytochalasin action on cells. Cytochalasin B or cytochalasin D rapidly disorganize the microfilament bundles and cause cell arborization. Microfilament bundle destruction in the cells incubated in the mixture of cytochalasin and any of the inhibitors requires 90 min and is not accompanied by dramatic changes in the cell morphology, so the process is indistinguishable from microfilament bundle destruction in the presence of the inhibitors alone.