Analysis of monodisperse, sequence-defined, and POSS-functionalized polyester copolymers by MALDI tandem mass spectrometry
Analysis of monodisperse, sequence-defined, and POSS-functionalized polyester copolymers by MALDI tandem mass spectrometry
复制标题
DOI：
10.1177/1469066719828875
复制
发表时间：
2019-02-01
影响因子：
1.3
通讯作者：
Wesdemiotis, Chrys
中科院分区：
文献类型：
--
作者：
Mao, Jialin;Zhang, Wei;Wesdemiotis, Chrys
单分散、序列定义的聚合物可以潜在地用于数字数据存储。本研究报道了多面体低聚硅氧烷（POSS）纳米颗粒携带侧链以特定顺序功能化的单分散聚酯共聚物的序列分析和区分。利用Steglich酯化反应和琥珀酸酐开环化学反应迭代合成目标序列，并用基质辅助激光解吸电离串联质谱（MALDI-MS2）对序列进行了表征。根据其不同的断裂模式，可以很容易地区分同分异构体。嵌入在低聚物中的序列通过其特定的骨干解离途径被解密。利用MALDI-MS2作为单分散合成大分子测序方法的稳健性通过对较长低聚物的表征进行了评估和验证。
Monodisperse, sequence-defined polymers can be potentially used for digital data storage. This study reports the sequence analysis and differentiation of monodisperse polyester copolymers carrying side chains functionalized in a specific order by polyhedral oligomeric silsesquioxane (POSS) nanoparticles. Steglich esterification and succinic anhydride ring-opening chemistries were utilized iteratively to synthesize the intended sequences, which were characterized by matrix-assisted laser desorption ionization tandem mass spectrometry (MALDI-MS2). Isomeric oligomers were readily distinguished based on their different fragmentation patterns. The sequences embedded in the oligomers were decrypted by their specific backbone dissociation pathways. The robustness of using MALDI-MS2 as a sequencing method for monodisperse synthetic macromolecules was assessed and validated by the characterization of longer oligomers.