Rapid high-performance liquid chromatographic method for the measurement of amiodarone in blood plasma or serum at the concentrations attained during therapy.
Rapid high-performance liquid chromatographic method for the measurement of amiodarone in blood plasma or serum at the concentrations attained during therapy.
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快速高效液相色谱方法，用于测量血浆或血清中治疗期间达到的浓度的胺碘酮。
DOI：
10.1016/s0021-9673(00)80471-9
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发表时间：
1980
影响因子：
4.1
通讯作者：
Dw Holt
中科院分区：
文献类型：
--
作者：
R. Flanagan;G. Storey;Dw Holt
本文建立了测定小体积（200 μl）血浆或血清中抗心律失常药物胺碘酮的高效液相色谱法。加入含有内标物的2 mol/l磷酸盐溶液（pH 4.5，20 μl）后，将样品与二异丙醚（200 μl）涡旋混合30秒。在微粒（5 μm）硅胶柱上使用含高氯酸（0.02% v/v）的甲醇-乙醚（85：15）作为移动的流动相分析所得提取物的一部分（100 μl），并在240 nm处监测柱流出物的吸收。未观察到内源性干扰源，其他药物的干扰极小。该程序是快速的，一式两份的分析需要不到15分钟完成。测定的灵敏度限度为0.05 mg/l，在接受该化合物治疗的患者血浆样本中测得的胺碘酮浓度范围为0.15 - 4.5 mg/l。
A simple high-performance liquid chromatographic method has been developed for the measurement of the antiarrhythmic drug amiodarone in small (200 μl) volumes of plasma or serum. After addition of 2 mole/1 phosphate solution, pH 4.5 (20 μl), containing the internal standard, the sample is vortex-mixed with diisopropyl ether (200 μl) for 30 sec. A portion (100 μl) of the resulting extract is analysed on a microparticulate (5 μm) silica column using methanol-diethyl ether (85:15) containing perchloric acid (0.02% v/v) as the mobile phase, and the absorption of the column effluent is monitored at 240 nm. No endogenous sources of interference have been observed, and interference from other drugs is minimal. The procedure is rapid, an analysis in duplicate taking less than 15 min to complete. The limit of sensitivity of the assay is 0.05 mg/l, and the concentrations of amiodarone measured in plasma samples from patients under treatment with this compound ranged from 0.15 to 4.5 mg/l.