Extensive characterization of human tear fluid collected using different techniques unravels the presence of novel lipid amphiphiles
Extensive characterization of human tear fluid collected using different techniques unravels the presence of novel lipid amphiphiles
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DOI：
10.1194/jlr.m044826
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发表时间：
2014-02-01
影响因子：
6.5
通讯作者：
Shui, Guanghou
中科院分区：
文献类型：
--
作者：
Lam, Sin Man;Tong, Louis;Shui, Guanghou
泪膜覆盖前眼，其各种组成成分的精确平衡对于维持眼部健康至关重要。特别是泪膜两亲性脂质亚层的组成，由于泪液中这些脂质两亲物的浓度有限，使得它们的检测和准确定量繁琐，因此在很大程度上仍然是一个有争议的问题。使用系统和敏感的脂质组学方法，我们验证了不同的泪液收集技术，并报告了迄今为止最全面的人类泪液脂质组;包括来自17个主要脂质类的600多种脂质。我们的研究赋予新的见解，现有的泪膜模型的组成细节，特别是有争议的两亲性脂质亚层成分，通过证明存在的胆固醇硫酸酯，O-酰基-ω-羟基脂肪酸，和各种鞘脂和磷脂的眼泪。本文报道的各种泪液脂质两亲物的相对丰度的发现和定量预期对现有人类泪膜模型的当前理解具有深远影响。
The tear film covers the anterior eye and the precise balance of its various constituting components is critical for maintaining ocular health. The composition of the tear film amphiphilic lipid sublayer, in particular, has largely remained a matter of contention due to the limiting concentrations of these lipid amphiphiles in tears that render their detection and accurate quantitation tedious. Using systematic and sensitive lipidomic approaches, we validated different tear collection techniques and report the most comprehensive human tear lipidome to date; comprising more than 600 lipid species from 17 major lipid classes. Our study confers novel insights to the compositional details of the existent tear film model, in particular the disputable amphiphilic lipid sublayer constituents, by demonstrating the presence of cholesteryl sulfate, O-acyl-omega-hydroxy fatty acids, and various sphingolipids and phospholipids in tears. The discovery and quantitation of the relative abundance of various tear lipid amphiphiles reported herein are expected to have a profound impact on the current understanding of the existent human tear film model.