Stretching of homopolymeric RNA reveals single-stranded helices and base-stacking
Stretching of homopolymeric RNA reveals single-stranded helices and base-stacking
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DOI：
10.1103/physrevlett.98.158103
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发表时间：
2007-04-13
影响因子：
8.6
通讯作者：
Halperin, Avraham
中科院分区：
文献类型：
--
作者：
Seol, Yeonee;Skinner, Gary M.;Halperin, Avraham
我们通过拉伸聚腺苷酸[poly（A）]和聚胞苷酸[poly（C）]的单个分子，发现了单链核酸螺旋结构的有力支持证据。分析力与扩展数据使用两个状态的弹性模型，其中随机线圈域交替刚性螺旋域允许一个提取的热力学和结构特性。此外，它还分别产生了poly（A）和poly（C）的螺旋-卷曲转变的中等至低的协同性。
We have found strong supporting evidence for the helical structures of single-stranded nucleic acids by stretching individual molecules of polyadenylic acid [poly(A)] and polycytidylic acid [poly(C)]. Analyzing the force versus extension data using a two-state elastic model in which random-coil domains alternate with rigid helical domains allows one to extract the thermodynamic and structural properties. In addition, it also yields moderate to low cooperativity of the helix-coil transition for poly(A) and poly(C), respectively.