ANALYSIS OF THE CREA GENE, A REGULATOR OF CARBON CATABOLITE REPRESSION IN ASPERGILLUS-NIDULANS
ANALYSIS OF THE CREA GENE, A REGULATOR OF CARBON CATABOLITE REPRESSION IN ASPERGILLUS-NIDULANS
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DOI：
10.1128/mcb.11.11.5701
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发表时间：
1991-11-01
影响因子：
5.3
通讯作者：
KELLY, JM
中科院分区：
文献类型：
--
作者：
DOWZER, CEA;KELLY, JM
完整的核苷酸序列来自构巢曲霉creA基因的基因组克隆和两个cDNA克隆。 该基因不含内含子。 衍生的415个氨基酸的多肽含有两个C2H2类的锌指，常见的S（T）PXX基序，和一个富含丙氨酸的区域指示的DNA结合阻遏蛋白。 锌指区的氨基酸序列与Mig1的锌指区具有84%的相似性，Mig1是一种参与酵母细胞中碳分解代谢物阻遏的蛋白质，并且它与哺乳动物Egr 1和Egr 2蛋白以及Wilms肿瘤蛋白都相关。 删除删除creA基因获得，通过使用体外技术，在异核体和二倍体菌株，但在纯的单倍体条件下是无法获得的。 有证据表明，当没有另一个creA等位基因补充时，这种缺失的表型是泄漏致死性的，允许孢子的有限萌发，但不允许菌落形成。 这种表型比任何体内产生的突变都要极端得多，因此基因产物可能具有激活剂活性以及阻遏物功能，或者完全存活可能需要一些残留的阻遏物功能。
The complete nucleotide sequence derived from a genomic clone and two cDNA clones of the creA gene of Aspergillus nidulans is presented. The gene contains no introns. The derived polypeptide of 415 amino acids contains two zinc fingers of the C2H2 class, frequent S(T)PXX motifs, and an alanine-rich region indicative of a DNA-binding repressor protein. The amino acid sequence of the zinc finger region has 84% similarity to the zinc finger region of Mig1, a protein involved in carbon catabolite repression in yeast cells, and it is related both to the mammalian Egr1 and Egr2 proteins and to the Wilms' tumor protein. A deletion removing the creA gene was obtained, by using in vitro techniques, in both a heterokaryon and a diploid strain but was unobtainable in a pure haploid condition. Evidence is presented suggesting that the phenotype of such a deletion, when not complemented by another creA allele, is leaky lethality allowing limited germination of the spore but not colony formation. This phenotype is far more extreme than that of any of the in vivo-generated mutations, and thus either the gene product may have an activator activity as well as a repressor function or some residual repressor function may be required for full viability.