Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia.
Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia.
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在没有临床疾病的牛和布法罗在东南亚的临床疾病的情况下检测脚和口疾病病毒。
DOI：
10.3389/fvets.2021.691308
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发表时间：
2021
影响因子：
3.2
通讯作者：
Mioulet V
中科院分区：
文献类型：
--
作者：
Buckle K;Bueno R;McFadden A;van Andel M;Spence R;Hamill C;Roe W;Vallee E;Castillo-Alcala F;Abila R;Verin B;Purevsuren B;Sutar A;Win HH;Thiha M;Lwin KO;Khounsy S;Phonthasy S;Souriya V;Keokhamphet C;Arzt J;Ludi A;Mioulet V
口蹄疫病毒（FMDV）在世界大部分地区广泛传播，包括东南亚部分地区。监测往往局限于流行地区，主要依靠被动的疫情报告。作为世界动物卫生组织（OIE）东南亚和中国口蹄疫项目（SEACFMD）的一部分，进行了现场采样，以帮助了解以前研究中观察到的广泛病毒暴露的证据。在三个屠宰场屠宰后立即收集血清和干粘膜拭子，以评估262头健康牛（老挝n = 84;缅甸n = 125）和布法罗（老挝n = 48;缅甸n = 5）鼻、口和背鼻咽粘膜表面上FMDV RNA的存在。拭子和血清由OIE/FAO口蹄疫世界参考实验室（WRLFMD）使用全血清型实时逆转录-PCR（rRT-PCR）进行检测，血清使用FMD Prioprotein非结构蛋白（NSP）ELISA进行评价。总共有7.3%的动物在三个部位中的一个或多个部位检测到FMDV RNA，包括5.3%的鼻咽拭子，2.3%的口腔拭子和1.5%的鼻拭子。血清中未检测到FMDV RNA。总体而言，37.8%的动物对NSP抗体呈阳性，表明可能过去自然暴露于FMDV。老挝和缅甸以及牛和布法罗的结果具有可比性，年龄组之间无显著差异。在老挝和缅甸的临床健康的大型反刍动物的口腔和鼻粘膜上存在可检测的FMDV RNA，这证明了作为监测的一部分对无症状动物进行采样的重要性，并可能表明亚临床感染在这些国家的FMD流行病学中起作用。
Foot-and-mouth disease virus (FMDV) is widespread throughout much of the world, including parts of South East Asia. Surveillance is often limited in endemic areas, relying predominantly on passive outbreak reporting. As part of the World Organisation for Animal Health (OIE)'s South East Asia and China Foot-and-Mouth Disease Project (SEACFMD), field sampling was performed to help understand evidence of widespread virus exposure observed in previous studies. Serum and dry mucosal swabs were collected to evaluate the presence of FMDV RNA on the nasal, oral, and dorsal nasopharyngeal mucosal surfaces of 262 healthy cattle (n = 84 in Laos; n = 125 in Myanmar) and buffalo (n = 48 in Laos; n = 5 in Myanmar) immediately following slaughter in three slaughterhouses. Swabs and serum were tested by the OIE/FAO World Reference Laboratory for foot-and-mouth disease (WRLFMD) using pan-serotypic real-time reverse transcription-PCR (rRT-PCR) and serum was evaluated using the FMD PrioCHECK non-structural protein (NSP) ELISA. In total, 7.3% of animals had detectable FMDV RNA in one or more of the three sites including 5.3% of nasopharyngeal swabs, 2.3% of oral swabs, and 1.5% of nasal swabs. No FMDV RNA was detected in serum. Overall, 37.8% of animals were positive for NSP antibodies, indicating likely past natural exposure to FMDV. Results were comparable for Laos and Myanmar, and for both cattle and buffalo, and were not significantly different between age groups. Detectable FMDV RNA present on the oral and nasal mucosa of clinically-healthy large ruminants in Laos and Myanmar demonstrates the importance of sampling asymptomatic animals as part of surveillance, and may indicate that subclinical infection plays a role in the epidemiology of FMD in these countries.
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影响因子：
3.7
作者：
Biswal, Jitendra K.;Ranjan, Rajeev;Arzt, Jonathan
通讯作者：
Arzt, Jonathan
DOI：
10.2460/javma.2002.220.1636
发表时间：
2002-06-01
期刊：
JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
影响因子：
--
作者：
Callahan, JD;Brown, F;Nelson, WM
通讯作者：
Nelson, WM
影响因子：
4.6
作者：
Bronsvoort, Barend M. deC.;Handel, Ian G.;Morgan, Kenton L.
通讯作者：
Morgan, Kenton L.
影响因子：
2.6
作者：
Bertram, Miranda R.;Delgado, Amy;Arzt, Jonathan
通讯作者：
Arzt, Jonathan
影响因子：
1.5
作者：
King, DP;Ferris, NP;Beckham, TR
通讯作者：
Beckham, TR