Characterization of Arsenic Biotransformation by a Typical Bryophyte Physcomitrella patens.
Characterization of Arsenic Biotransformation by a Typical Bryophyte Physcomitrella patens.
复制标题
典型苔藓植物小立碗藓 (Physcomitrella patens) 砷生物转化的表征。
DOI：
10.1007/s00128-016-1997-y
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发表时间：
2017
影响因子：
2.7
通讯作者：
Gao Jianwei
中科院分区：
文献类型：
--
作者：
Yin Xixiang;Wang Lihong;Liu Yifei;Jiang Tenglong;Gao Jianwei
砷（As）是一种普遍存在的环境毒素，在全球范围内造成了严重的人类健康和环境问题，而白立碗藓（Physcomitrella patensis）是一种潜在的环境监测模式植物，广泛存在于各种生态系统中。在这项研究中，研究了这种苔藓的砷代谢。在不同浓度（50、100、200 μmol/L）的砷酸盐处理4周后，该苔藓中总砷含量高达231.4-565.4 mg/kg DW。砷的含量随外源砷浓度的增加而增加，占总砷的25.1-36.8%。一个砷酸还原酶，PpACR 2，进行了鉴定和功能表征。大肠杆菌对砷（V）敏感株WC 3110（ΔarsC）中PpACR 2的异源表达增强了对砷（V）的抗性。纯化的PpACR 2蛋白具有砷酸还原酶活性。由于其强大的砷积累能力，britonite可以开发的生物修复砷污染的环境。
Arsenic (As) is a ubiquitous environmental toxin that has created catastrophic human health and environmental problems around world.Physcomitrella patensis a potential model plant for the study of environmental monitoring, which exists in all kinds of ecosystems. In this study, arsenic metabolism was investigated by this moss. When supplied with different levels of arsenate (50, 100, 200 µmol/L) for a 4-week period, the total arsenic concentrations were up to 231.4–565.4 mg/kg DW in this moss. Arsenite concentration increased with increasing external arsenate concentrations, the proportion was up to 25.1–36.8% of the total As. An arsenate reductase, PpACR2, was identified and functionally characterized. Heterologous expression ofPpACR2in an As(V)-sensitive strain WC3110 (ΔarsC) ofEscherichia coliconferred As(V) resistance. Purified PpACR2 protein exhibited the arsenate reductase activity. Given its powerful As accumulation ability, the bryophyte could be exploited in bioremediation of As-contaminated environments.