FMT, a protein that affects mitochondrial distribution, interacts with translation-related proteins in Arabidopsis thaliana
FMT, a protein that affects mitochondrial distribution, interacts with translation-related proteins in Arabidopsis thaliana
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DOI：
10.1007/s00299-020-02634-9
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发表时间：
2021-01-01
影响因子：
6.2
通讯作者：
Arimura, Shin-ichi
中科院分区：
文献类型：
--
作者：
Ayabe, Hiroki;Kawai, Narumi;Arimura, Shin-ichi
关键信息两个翻译相关蛋白被鉴定为FMT相互作用蛋白。然而，与苍蝇和人类中其他CLU基因的突变不同，FMT对线粒体蛋白的积累没有明显的影响。细胞器的分布对有效的新陈代谢和胁迫反应是至关重要的，并受各种环境因素的控制。簇状线粒体(CLU)超家族基因影响线粒体的分布，在酵母、苍蝇、哺乳动物和拟南芥等多种物种中，CLU基因的破坏导致线粒体聚集在细胞内。在拟南芥中，友好线粒体(FMT)是线粒体正常分布所必需的CLU基因，但其分子功能尚不清楚。在这里，我们证明了FMT与一些翻译相关蛋白(翻译起始因子eIFiso4G1和谷氨酰-tRNA合成酶OVA9)以及自身的相互作用。我们还发现，FMT在胞浆中形成动态颗粒，有时与线粒体一起运动，它们的运动主要由肌动蛋白细丝控制，但也受微管控制。在动物CLU同源基因上也有类似的结果报道。然而，与动物CLU突变体不同，FMT突变体没有表现出任何明显的核编码线粒体蛋白水平的下降。这种差异可能反映了FMT与其他CLU超家族基因在功能上的差异。
Key message Two translation-related proteins are identified as FMT-interacting proteins. However, FMT, unlike mutants of other CLU genes in fly and human, has no clear impact on the accumulation of mitochondrial proteins. Organelle distribution is critical for effective metabolism and stress response and is controlled by various environmental factors. Clustered mitochondria (CLU) superfamily genes affect mitochondrial distribution and their disruptions cause mitochondria to cluster within a cell in various species including yeast, fly, mammals and Arabidopsis. In Arabidopsis thaliana, Friendly mitochondria (FMT) is a CLU gene that is required for normal mitochondrial distribution, but its molecular function is unclear. Here, we demonstrate that FMT interacts with some translation-related proteins (translation initiation factor eIFiso4G1 and glutamyl-tRNA synthetase OVA9), as well as itself. We also show FMT forms dynamic particles in the cytosol that sometimes move with mitochondria, and their movements are mainly controlled by actin filaments but also by microtubules. Similar results have been reported for animal CLU orthologs. However, an fmt mutant, unlike animal clu mutants, did not show any clear decrease of nuclear-encoded mitochondrial protein levels. This difference may reflect a functional divergence of FMT from other CLU superfamily genes.