Behavioral and histological characterization of unilateral cervical spinal cord contusion injury in rats
Behavioral and histological characterization of unilateral cervical spinal cord contusion injury in rats
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DOI：
10.1089/neu.2006.23.36
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发表时间：
2006-01-01
影响因子：
4.2
通讯作者：
Bresnahan, JC
中科院分区：
文献类型：
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作者：
Gensel, JC;Tovar, CA;Bresnahan, JC
大多数大鼠脊髓损伤（SCI）的实验研究损害胸髓，随后的功能丧失是由于连接尾脊髓和喙神经系统的长束中断。尽管颈髓是人类SCI最常见的水平，但评估颈髓损伤的工作较少。除了长束外，颈脊髓还包含负责上肢功能的感觉和运动神经元。本研究的目的是利用改良的NYU/MASCIS重物坠落装置进一步建立大鼠颈脊髓挫伤模型。造成轻度（6.25 mm）和中度（12.5 mm）C5单侧损伤。使用梳理测试、爪偏好测试、走道测试（The Catwalk）和水平梯测试来检查行为恢复。组织学结果测量包括损伤中心的保留、整个损伤范围的保留、损伤头侧和尾侧髓鞘丢失的定量以及运动神经元计数。与对照组相比，接受SCI的动物在SCI后6周内表现出前肢、运动和后肢功能的损伤严重程度特异性缺陷。组织学分析显示损伤的同侧包容性，以及除运动神经元计数外的所有指标的组间差异。该模型具有许多优点：（1）SCI后动物护理要求最低，（2）在受试者对照范围内，（3）功能丧失主要涉及同侧前肢，以及（4）它是挫伤性SCI引起的灰质和白色物质损伤的行为和组织学模型。
Most experimental studies of spinal cord injury (SCI) in rats damage the thoracic cord, with the consequent functional loss being due to interruption of long tracts connecting the caudal spinal cord to the rostral nervous system. Less work has been done evaluating injury to the cervical cord, even though it is the most common level of human SCI. In addition to the long tracts, the cervical spinal cord contains the sensory and motor neurons responsible for upper extremity function. The purpose of this study was to further develop a rat model of cervical spinal cord contusion injury using a modified NYU/MASCIS weight drop device. Mild (6.25 mm) and moderate (12.5 mm) C5 unilateral injuries were produced. Behavioral recovery was examined using a grooming test, a paw preference test, a walkway test (The Catwalk), and a horizontal ladder test. Histological outcome measures included sparing at the lesion epicenter, sparing throughout the extent of the lesion, quantification of myelin loss rostral and caudal to the lesion, and motor neuron counts. Compared to controls, animals receiving SCI exhibited injury severity-specific deficits in forelimb, locomotor, and hindlimb function persisting for 6-weeks post-SCI. Histological analysis revealed ipsilateral containment of the injury, and differentiation between groups on all measures except motor neuron counts. This model has many advantages: (1) minimal animal care requirements post-SCI, (2) within subject controls, (3) functional loss involves primarily the ipsilateral forelimb, and (4) it is a behavioral and histological model for both gray and white matter damage caused by contusive SCI.