Enhancement of neurite outgrowth in neuron cancer stem on 3-D collagen scaffolds
Enhancement of neurite outgrowth in neuron cancer stem on 3-D collagen scaffolds
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DOI：
10.1016/j.bbrc.2012.10.007
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发表时间：
2012-11-09
影响因子：
3.1
通讯作者：
Liu, Li-Feng
中科院分区：
文献类型：
--
作者：
Chen, Chih-Hao;Kuo, Shyh Ming;Liu, Li-Feng
Collagen is one component of the extracellular matrix that has been widely used for constructive remodeling to facilitate cell growth and differentiation. The 3-D distribution and growth of cells within the porous scaffold suggest a clinical significance for nerve tissue engineering. In the current study, we investigated proliferation and differentiation of neuron cancer stem cells (NCSCs) on a 3-D porous collagen scaffold that mimics the natural extracellular matrix. We first generated green fluorescence protein (GFP) expressing NCSCs using a lentiviral system to instantly monitor the transitions of morphological changes during growth on the 3-D scaffold. We found that proliferation of GFP-NCSCs increased, and a single cell mass rapidly grew with unrestricted expansion between days 3 and 9 in culture. Moreover, immunostaining with neuronal nuclei (NeuN) revealed that NCSCs grown on the 3-D collagen scaffold significantly enhanced neurite outgrowth. Our findings confirmed that the 80 mu m porous collagen scaffold could enhance attachment, viability and differentiation of the cancer neural stem cells. This result could provide a new application for nerve tissue engineering and nerve regeneration. (C) 2012 Elsevier Inc. All rights reserved.
