RNAi-mediated knocking-down of rlpk2 gene retarded soybean leaf senescence

RNAi-mediated knocking-down of rlpk2 gene retarded soybean leaf senescence
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RNAi介导的rlpk2基因敲除延缓大豆叶片衰老

DOI:
10.1360/982005-219
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发表时间:
2005-06-01
影响因子:
--
通讯作者:
Wang, NN
Wang, NN
中科院分区:
其他
文献类型:
--
作者:
Li, XP;Ma, YY;Wang, NN

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Leaf senescence that occurs in the last stage of leaf development is a genetically programmed process. It is very significant to elucidate the molecular mechanisms that control the initiation and progression of leaf senescence and the way the senescence signal is transduced. In a previous study on artificially induced soybean leaf senescence, we cloned a novel gene designated rlpk2 (Genbank Accession No. AY687391) that encodes a leucine-rich repeat (LRR) receptor like protein kinase. The expression level of rlpk2 gene was shown to be strongly up-regulated during both the natural leaf senescence process in this report and the artificially induced primary-leaf-senescence process in our previous work. The RNA interference (RNAi)-mediated knocking-down of rlpk2 dramatically retarded both the natural and nutrient deficiency-induced leaf senescence in transgenic soybean. The transgenic leaves showed more cell-aggregated surface structure and higher content of chlorophyll.