FURTHER-STUDIES ON THE DEGRADATION OF IMMUNOGLOBULINS BY BLACK-PIGMENTED BACTEROIDES
FURTHER-STUDIES ON THE DEGRADATION OF IMMUNOGLOBULINS BY BLACK-PIGMENTED BACTEROIDES
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DOI：
10.1111/j.1399-302x.1989.tb00400.x
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发表时间：
1989-03-01
影响因子：
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通讯作者：
MCBRIDE, BC
中科院分区：
文献类型：
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作者：
GRENIER, D;MAYRAND, D;MCBRIDE, BC
本研究证实了几种黑色素类杆菌对免疫球蛋白A和G的降解能力。免疫球蛋白的裂解产物强烈刺激了降解免疫球蛋白的细菌的生长。牙龈类杆菌在添加免疫球蛋白的限制性培养基上的生长与在完全培养基上的平行生长。黑色素类杆菌对Ig G和Ig A的降解分两个阶段进行。这些分子被分解成大片段，随后被降解成在SDS-PAGE上看不到的小肽。牙龈假单胞菌将免疫球蛋白降解为多肽，其相对分子质量分别为33,000和11,000，而解糖类杆菌、中间类杆菌和洛氏拟杆菌仅形成33,000个相对分子质量的多肽。含有共价连接的胰岛素样生长因子的聚丙烯酰胺凝胶的电泳法。免疫球蛋白和牛血清白蛋白结果表明，牙龈假单胞菌具有8种不同的蛋白水解酶活性。蛋白水解酶保护细胞不与抗B抗体反应。能降解细菌细胞表面结合的抗体。
The ability of several species of black-pigmented Bacteroides to degrade immunoglobulins A and G was confirmed in this study. The cleavage products from IgG strongly stimulated the growth of bacteria degrading IgG. Growth of Bacteroides gingivalis on limiting media supplemented with IgG paralleled growth on complete medium. The degradation of IgG and IgA by black-pigmented Bacteroides appeared to occur in 2 stages. The molecules were broken into large fragments which were subsequently degraded into small peptides not visible on SDS-PAGE. B. gingivalis degraded IgG to peptides with Mrs of 33,000 and 11,000 whereas Bacteroides asaccharolyticus, Bacteroides intermedius and Bacteroides loescheii formed only the 33,000 Mr peptide. Electrophoresis in polyacrylamide gels containing covalently linked IgF. IgA and bovine serum albumin revealed that B. gingivalis elaborated 8 electrophoretically distinct proteolytic activities. The proteases protected the cell from reaction with anti-B. gingivalis antibody and were capable of hydrolyzing antibody bound to the bacterial cell surface.