Collagen synthesis in human musculoskeletal tissues and skin
Collagen synthesis in human musculoskeletal tissues and skin
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DOI：
10.1152/ajpendo.00243.2005
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发表时间：
2005-11-01
影响因子：
5.1
通讯作者：
Rennie, MJ
中科院分区：
文献类型：
--
作者：
Babraj, JA;Cuthbertson, DJR;Rennie, MJ
我们开发了一种直接测量人类肌肉骨骼胶原蛋白合成的方法，该方法基于将稳定同位素标记的脯氨酸或亮氨酸掺入蛋白质中，并使用它来测量肌腱、韧带、肌肉中胶原蛋白的合成速率。和皮肤。在吸收后，健康年轻男性（28 ± 6岁）的肌腱、韧带、肌肉和皮肤胶原蛋白合成率分别为0.046 ± 0.005、0.040 ± 0.006、0.016 ± 0.002和0.037 ± 0.003%/ h（平均值± SD）。在吸收后的健康老年男性（70 +/- 6岁）中，骨骼肌胶原蛋白合成速率大于年轻人（0.023 +/- 0.002%/ h，P < 0.05 vs.年轻人）。肌腱和韧带胶原蛋白的合成速率与吸收后状态下混合骨骼肌蛋白的合成速率相似，而年轻和老年男性的肌肉胶原蛋白合成速率要低得多。营养供应后，肌腱和骨骼肌的胶原蛋白合成没有改变，保持在吸收后值（年轻：肌腱，0.045 +/- 0.008%/ h;肌肉，0.016 +/- 0.003%/ h;老年：肌肉，0.024 +/- 0.003%/ h）。这些结果表明，人类肌肉骨骼组织胶原合成的速率可以直接和鲁棒地使用稳定同位素方法测量。
We have developed a direct method for the measurement of human musculoskeletal collagen synthesis on the basis of the incorporation of stable isotope-labeled proline or leucine into protein and have used it to measure the rate of synthesis of collagen in tendon, ligament, muscle, and skin. In postabsorptive, healthy young men ( 28 +/- 6 yr) synthetic rates for tendon, ligament, muscle, and skin collagen were 0.046 +/- 0.005, 0.040 +/- 0.006, 0.016 +/- 0.002, and 0.037 +/- 0.003%/ h, respectively ( means +/- SD). In postabsorptive, healthy elderly men ( 70 +/- 6 yr) the rate of skeletal muscle collagen synthesis is greater than in the young (0.023 +/- 0.002%/ h, P < 0.05 vs. young). The rates of synthesis of tendon and ligament collagen are similar to those of mixed skeletal muscle protein in the postabsorptive state, whereas the rate for muscle collagen synthesis is much lower in both young and elderly men. After nutritient provision, collagen synthesis was unaltered in tendon and skeletal muscle, remaining at postabsorptive values ( young: tendon, 0.045 +/- 0.008%/ h; muscle, 0.016 +/- 0.003%/ h; elderly: muscle, 0.024 +/- 0.003%/ h). These results demonstrate that the rate of human musculoskeletal tissue collagen synthesis can be directly and robustly measured using stable isotope methodology.