Plasmid DNA and IL-4 modulate expression of mHC class I and costimulatory molecules in B lymphocytes.
Plasmid DNA and IL-4 modulate expression of mHC class I and costimulatory molecules in B lymphocytes.
复制标题
质粒 DNA 和 IL-4 调节 B 淋巴细胞中 mHC I 类和共刺激分子的表达。
DOI：
10.1089/dna.2006.0539
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发表时间：
2007
影响因子：
3.1
通讯作者：
Zanetti,Maurizio
中科院分区：
文献类型：
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作者：
Frazzi,Raffaele;Zanetti,Maurizio
B淋巴细胞能够自发地内化质粒(P)DNA，这一事件启动了这类造血细胞的抗原呈递功能。在此之前，我们已经证明，自发转基因B淋巴细胞启动CD8 T淋巴细胞需要T细胞的帮助，而这可以被可溶性IL-4所替代。为了更好地理解这一现象，我们研究了PDNA和IL-4对MHC-I和一组关键的共刺激分子(CD40、CD80、CD86、OX40L和LAG-3)表达的相对作用。虽然MHC-I的上调是由PDNA贡献的，但IL-4主要上调CD86，少量上调CD40。这两种影响似乎是独立的。此外，还发现IL-4能稳定pDNA自发转基因后淋巴细胞中MHC-I的转录。这些结果进一步加深了我们对暴露于pDNA后在特定哺乳动物细胞中发生的事件的理解。他们还指出，在PDNA内化后，B淋巴细胞的抗原呈递功能可以被IL-4补充，IL-4是一种通常由激活的CD4T细胞产生的细胞因子。
B lymphocytes are capable of spontaneous internalization of plasmid (p)DNA, an event that set in motion the antigen-presenting function in this class of hemopoietic cells. Previously, we showed that priming of CD8 T lymphocytes by spontaneously transgenic B lymphocytes requires T-cell help, and that this can be replaced by soluble IL-4. To better understand this phenomenon we studied the relative role of pDNA and IL-4 on the expression of MHC-I and a panel of critical costimulatory molecules (CD40, CD80, CD86, OX40L, and LAG-3). Whereas upregulation of MHC-I is contributed by pDNA, IL-4 mainly upregulates CD86 and to a lesser degree CD40. The two effects appear to be independent. In addition, however, it was found that IL-4 stabilizes MHC-I transcription in lymphocytes after spontaneous transgenesis with pDNA. These results further our understanding of events that take place in specialized mammalian cells after exposure to pDNA. They also point to the fact after pDNA internalization that the antigen-presenting function of B lymphocytes can be complemented by IL-4, a cytokine normally produced by activated CD4 T cells.