Conformational Switching of Pyrenes Associated on Hairpin Loop Region by DNA B‐Z Transition
Conformational Switching of Pyrenes Associated on Hairpin Loop Region by DNA B‐Z Transition
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DOI：
10.1002/slct.202200696
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发表时间：
2022-08
期刊：
影响因子：
2.1
通讯作者：
Mitsunobu Nakamura;Hibiki Yoshioka;T. Takada
中科院分区：
文献类型：
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作者：
Mitsunobu Nakamura;Hibiki Yoshioka;T. Takada
DNA B−Z 跃迁在生物系统中发挥着重要作用，并在 DNA 纳米技术中引起了广泛关注。 DNA 及其类似物也被用作模板来构建具有 π 相互作用的螺旋发色团。我们制备了在 d(CG) 重复序列中间具有连续非核苷芘残基的 DNA。芘标记的DNA采用单分子发夹结构，其中芘在环区域结合。他们表现出激子耦合圆二色性信号的反转，这可归因于通过 DNA B−Z 跃迁产生的芘缔合。这表明芘缔合物的构象可以通过 DNA B−Z 转变从右向左转换。当切换关联时，芘标记的 DNA 的荧光发生变化。因此，芘标记的DNA可以用作手性光学分子开关。
DNA B−Z transitions play an important role in biological systems and have attracted much attention in DNA nanotechnology. DNA and its analogues have also been used as templates to construct helical chromophore associates with π‐interactions. We prepared DNAs with consecutive non‐nucleoside pyrene residues in the middle of d(CG) repeat sequences. The pyrene‐labelled DNAs adopted a unimolecular hairpin structure, in which the pyrenes associated in the loop region. They exhibited inversion of exciton‐coupled circular dichroism signals that can be ascribed to pyrene association through a DNA B−Z transition. This indicates that the conformation of pyrene associates can be switched from right to left by the DNA B−Z transition. The fluorescence of the pyrene‐labelled DNA changed when switching the association. Thus, pyrene‐labelled DNA can be utilized as a chiroptical molecular switch.