Subamolide E from Cinnamomum subavenium Induces Sub-G1 Cell-Cycle Arrest and Caspase-Dependent Apoptosis and Reduces the Migration Ability of Human Melanoma Cells
Subamolide E from Cinnamomum subavenium Induces Sub-G1 Cell-Cycle Arrest and Caspase-Dependent Apoptosis and Reduces the Migration Ability of Human Melanoma Cells
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DOI：
10.1021/jf2018929
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发表时间：
2011-08-10
影响因子：
6.1
通讯作者：
Chen, Chung-Yi
中科院分区：
文献类型：
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作者：
Wang, Hui-Min;Chiu, Chien-Chih;Chen, Chung-Yi
本工作的目的是研究天然化合物subamolide E对人皮肤癌黑色素瘤A375.S2细胞的抗癌细胞毒作用。 Subamolide E 是从 Cinnamomum subavenium 中分离出来的，并在 24 小时浓度范围为 0 至 100 μM 的细胞生长测定中显示出细胞毒性。采用碘化丙啶染色和流式细胞术分析评估细胞周期分布，发现subamolide E在治疗24小时后以剂量依赖性方式在亚G1期引起DNA损伤。 Western blot结果显示，subamolide-E处理的细胞中caspase依赖性凋亡蛋白的增加诱导了相关的通路机制。在伤口愈合试验中，Subamolide E 还显示出 A375.S2 细胞的抗迁移活性。最后，subamolide E 对正常人类皮肤细胞（角质形成细胞、黑素细胞和成纤维细胞）表现出轻微的细胞毒性；因此，它是一种潜在的针对皮肤黑色素瘤的化疗剂。
The aim of this work was to investigate the anticancer cytotoxic effects of natural compound subamolide E on the human skin cancer melanoma A375.S2 cells. Subamolide E was isolated from Cinnamomum subavenium and demonstrated cytotoxicities in the cell-growth assay at concentration ranges from 0 to 100 mu M at 24 h. Propidium iodide staining and flow cytometry analyses were used to evaluate cell-cycle distribution and found that subamolide E caused DNA damage in the sub-G1 phase with a dose-dependent manner after 24 h of treatment. According to the western blot result, subamolide-E-treated cells with the increase of caspase-dependent apoptotic proteins induced related pathway mechanisms. Subamolide E also showed antimigratory activities of A375.S2 cells on the wound-healing assay. Finally, subamolide E demonstrated minor cytotoxicities to normal human skin cells (keratinocytes, melanocytes, and fibroblasts); therefore, it is a potential chemotherapeutic agent against skin melanoma.