SSN6-TUP1 IS A GENERAL REPRESSOR OF TRANSCRIPTION IN YEAST
SSN6-TUP1 IS A GENERAL REPRESSOR OF TRANSCRIPTION IN YEAST
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DOI：
10.1016/0092-8674(92)90146-4
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发表时间：
1992-02-21
期刊：
影响因子：
64.5
通讯作者：
JOHNSON, AD
中科院分区：
文献类型：
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作者：
KELEHER, CA;REDD, MJ;JOHNSON, AD
同源结构域蛋白 α-2 和 SRF 样蛋白 Mcm1 是在酿酒酵母中建立细胞类型所必需的。这些调节蛋白共同识别特定的 DNA 操纵子，标记一组转录抑制基因。在本文中，我们表明 alpha-2-Mcm1 对算子的占据不足以带来抑制。相反，只有当细胞中也存在 Ssn6（一种 TPR 蛋白）和 Tup1（一种 β-转导蛋白重复蛋白）时，抑制才会生效。我们发现，当 Ssn6 通过细菌 DNA 结合结构域带到启动子时会抑制转录，而 Tup1 是这种抑制所必需的。基于这些和其他结果，我们提出 Ssn6-Tup1 是酵母中转录的通用阻遏蛋白，通过多种序列特异性 DNA 结合蛋白招募到目标启动子。
The homeodomain protein alpha-2 and the SRF-like protein Mcm1 are required to establish cell type in the yeast Saccharomyces cerevisiae. Together, these regulatory proteins recognize a specific DNA operator, marking a set of genes for transcriptional repression. In this paper, we show that occupancy of the operator by alpha-2-Mcm1 is not sufficient to bring about repression. Rather, repression is effected only when Ssn6 (a TPR protein) and Tup1 (a beta-transducin repeat protein) are also present in the cell. We show that Ssn6 represses transcription when brought to a promoter by a bacterial DNA-binding domain and that Tup1 is required for this repression. Based on these and other results, we propose that Ssn6-Tup1 is a general repressor of transcription in yeast, recruited to target promoters by a variety of sequence-specific DNA-binding proteins.