Tissue-engineered bone using mesenchymal stem cells and a biodegradable scaffold
Tissue-engineered bone using mesenchymal stem cells and a biodegradable scaffold
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DOI：
10.1097/00001665-200203000-00009
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发表时间：
2002-03-01
影响因子：
0.9
通讯作者：
Ueda, M
中科院分区：
文献类型：
--
作者：
Boo, JS;Yamada, Y;Ueda, M
骨髓已被证明含有一群稀有细胞，能够分化为形成各种组织的细胞。这些细胞被称为间充质干细胞(MSCs)，当异位移植到合适的支架中时，能够形成骨。本研究的目的是探讨新型β-磷酸三钙(β-TCP)作为支架材料的可能性，并比较其与羟基磷灰石(HA)的成骨能力。将负载MSCs的β-磷酸三钙和羟基磷灰石植入皮下，分别于植入后1、2、4、8周取材进行生化和组织学分析。生化检测显示，在β-磷酸三钙和羟基磷灰石复合材料中均能检测到碱性磷酸酶的活性，并能在8周内保持较高的活性。在组织学分析中，在β-磷酸三钙和羟基磷灰石复合材料中都可以发现活跃的骨形成。这些发现表明，β-TCP既可以作为支架，也可以作为HA的作用。利用可生物降解的β-磷酸三钙作为体内支架材料制成的人工骨可用于骨的重建，因为支架材料在植入几个月后被吸收。
Bone marrow has been shown to contain a population of rare cells capable of differentiating to the cells that form various tissues. These cells, referred to as mesenchymal stem cells (MSCs), are capable of forming bone when implanted ectopically in an appropriate scaffold. The aim of this study was to investigate the potential of a new beta-tricalcium phosphate (beta-TCP) as a scaffold and to compare the osteogenic potential between beta-TCP and hydroxyapatite (HA). The beta-TCP and HA loaded with MSCs were implanted in subcutaneous sites and harvested at 1, 2, 4, and 8 weeks after implantation for biochemical and histological analysis. Biochemically, in both beta-TCP and HA composites, the alkaline phosphatase activity in the composites could be detected and was maintained at a high level for 8 weeks. In the histological analysis, active bone formation could be found in both the beta-TCP and HA composites. These findings suggest that beta-TCP could play a role as a scaffold as well as HA. The fabricated synthetic bone using biodegradable beta-TCP as a scaffold in vivo is useful for reconstructing bone, because the scaffold material is absorbed several months after implantation.