The temporal transcriptomic response of Pinus massoniana seedlings to phosphorus deficiency.
The temporal transcriptomic response of Pinus massoniana seedlings to phosphorus deficiency.
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马尾松幼苗对缺磷的时间转录组响应
DOI：
10.1371/journal.pone.0105068
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wen X
中科院分区：
文献类型：
--
作者：
Fan F;Cui B;Zhang T;Qiao G;Ding G;Wen X
背景磷是植物生长发育所必需的大量营养元素。已经在各种植物物种中鉴定了参与缺磷胁迫的几个基因。然而，整个基因组的理解，参与植物适应低磷的分子机制仍然难以捉摸，特别是在针叶树这些驯化的遗传基础上的信息很少。马尾松（Pinusmassoniana）主要生长在我国热带和亚热带地区，其中许多地区严重缺乏无机磷（Pi）。在以前的工作中，我们描述了一个优良的马尾松基因型表现出高耐受性的Pi缺乏。方法/主要发现为了进一步研究耐低磷的机制，进行RNA-seq以给出来自两个混合文库的表达程度的概念，并使用基于从RNA-seq获得的unigenes设计探针的微阵列来阐明长期磷饥饿的全局基因表达谱。从来自RNA-Seq文库的112，108，862个高质量读段组装了长度范围为201至20，490 bp的总共70，896个单基因。我们分别鉴定了在P1（0.01 mM P）和P2（0.06 mM P）Pi缺乏条件下差异调节（P<0.05）的1，396和943个转录本。在磷缺乏胁迫下，许多转录本的表达水平一直受到差异调节，其中许多转录本在其他物种中也受到相应条件下的上调或下调，因此它们是监测植物磷营养状况的理想候选基因。研究结果还表明，不同磷饥饿水平对马尾松基因表达的影响。结论/意义据我们所知，这项工作提供了第一个深入了解的分子机制，参与驯化长期磷饥饿和不同的磷可用性水平的针叶树。
Background Phosphorus (P) is an essential macronutrient for plant growth and development. Several genes involved in phosphorus deficiency stress have been identified in various plant species. However, a whole genome understanding of the molecular mechanisms involved in plant adaptations to low P remains elusive, and there is particularly little information on the genetic basis of these acclimations in coniferous trees. Masson pine (Pinus massoniana) is grown mainly in the tropical and subtropical regions in China, many of which are severely lacking in inorganic phosphate (Pi). In previous work, we described an elite P. massoniana genotype demonstrating a high tolerance to Pi-deficiency. Methodology/Principal Findings To further investigate the mechanism of tolerance to low P, RNA-seq was performed to give an idea of extent of expression from the two mixed libraries, and microarray whose probes were designed based on the unigenes obtained from RNA-seq was used to elucidate the global gene expression profiles for the long-term phosphorus starvation. A total of 70,896 unigenes with lengths ranging from 201 to 20,490 bp were assembled from 112,108,862 high quality reads derived from RNA-Seq libraries. We identified 1,396 and 943 transcripts that were differentially regulated (P<0.05) under P1 (0.01 mM P) and P2 (0.06 mM P) Pi-deficiency conditions, respectively. Numerous transcripts were consistently differentially regulated under Pi deficiency stress, many of which were also up- or down-regulated in other species under the corresponding conditions, and are therefore ideal candidates for monitoring the P status of plants. The results also demonstrated the impact of different Pi starvation levels on global gene expression in Masson pine. Conclusions/Significance To our knowledge, this work provides the first insight into the molecular mechanisms involved in acclimation to long-term Pi starvation and different Pi availability levels in coniferous trees.
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影响因子：
11.6
作者：
Hamburger, D;Rezzonico, E;Poirier, Y
通讯作者：
Poirier, Y
影响因子：
11.6
作者：
Chen, Yi-Fang;Li, Li-Qin;Wu, Wei-Hua
通讯作者：
Wu, Wei-Hua
影响因子：
14.9
作者：
Harris, MA;Clark, J;White, R
通讯作者：
White, R
DOI：
10.1093/dnares/dss037
发表时间：
2013-04
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Hackenberg M;Huang PJ;Huang CY;Shi BJ;Gustafson P;Langridge P
通讯作者：
Langridge P
影响因子：
7.4
作者：
Baek, Dongwon;Kim, Min Chul;Yun, Dae-Jin
通讯作者：
Yun, Dae-Jin