Porcine circovirus type 2 increases interleukin-1beta and interleukin-10 production via the MyD88-NF-kappa B signaling pathway in porcine alveolar macrophages in vitro.
Porcine circovirus type 2 increases interleukin-1beta and interleukin-10 production via the MyD88-NF-kappa B signaling pathway in porcine alveolar macrophages in vitro.
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DOI：
10.4142/jvs.2017.18.2.183
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发表时间：
2017-06-30
影响因子：
1.8
通讯作者：
Lv Y
中科院分区：
文献类型：
--
作者：
Han J;Zhang S;Zhang Y;Chen M;Lv Y
猪肺泡巨噬细胞（PAM）是猪圆环病毒2型（PCV 2）经呼吸道感染后猪肺的第一道防线。然而，PCV 2感染损害PAM的杀微生物能力并改变细胞因子产生和/或分泌。目前，细胞因子失衡的原因尚未完全阐明，涉及的调控机制也不清楚。在本研究中，我们研究了白细胞介素-1 β（IL-1β）和IL-10在体外与PCV 2孵育后PAM中的表达水平和调控。与PCV 2共同孵育后，PAM培养上清中IL-1β和IL-10水平升高，核因子κ B（NF-κB）p65染色在细胞核中的分布、MyD 88和p-IκB在细胞浆中的表达以及NF-κB的DNA结合活性升高，而PAM细胞浆中p65的表达降低。然而，当PAM与PCV 2和靶向MyD 88的小干扰RNA共孵育时，这些作用被逆转。此外，当PAM与PCV 2孵育时，Toll样受体（TLR）-2、-3、-4、-7、-8和-9的mRNA表达水平增加。这些结果表明，PCV 2诱导PAM中IL-1β和IL-10产生增加，并且这些表达变化与TLR-MyD 88-NF-κB信号通路相关。
Porcine alveolar macrophages (PAMs) represent the first line of defense in the porcine lung after infection with porcine circovirus type 2 (PCV2) via the respiratory tract. However, PCV2 infection impairs the microbicidal capability of PAMs and alters cytokine production and/or secretion. At present, the reason for the imbalance of cytokines has not been fully elucidated, and the regulatory mechanisms involved are unclear. In this study, we investigated the expression levels and regulation of interleukin-1beta (IL-1β) and IL-10 in PAMs following incubation with PCV2 in vitro. Levels of IL-1β and IL-10 increased in PAM supernatants, and the distribution of nuclear factor kappa B (NF-κB) p65 staining in nucleus, expression of MyD88 and p-IκB in cytoplasm, and DNA-binding activity of NF-κB increased after incubation with PCV2, while p65 expression in PAM cytoplasm decreased. However, when PAMs were co-incubated with PCV2 and small interfering RNA targeting MyD88, those effects were reversed. Additionally, mRNA expression levels of Toll-like receptors (TLR)-2, -3, -4, -7, -8, and -9 increased when PAMs were incubated with PCV2. These results show that PCV2 induces increased IL-1β and IL-10 production in PAMs, and these changes in expression are related to the TLR–MyD88–NF-κB signaling pathway.