CELL AUTONOMOUS EXPRESSION OF IGD IS NOT ESSENTIAL FOR THE MATURATION OF CONVENTIONAL B-CELLS
CELL AUTONOMOUS EXPRESSION OF IGD IS NOT ESSENTIAL FOR THE MATURATION OF CONVENTIONAL B-CELLS
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DOI：
10.1093/intimm/3.12.1367
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发表时间：
1991-12-01
影响因子：
4.4
通讯作者：
RAJEWSKY, K
中科院分区：
文献类型：
--
作者：
ROES, J;RAJEWSKY, K
为了分析体内IgD的功能，我们利用基因靶向技术建立了一个“功能丧失”小鼠模型。通过同源重组（C57BL/6 × CBA）F1小鼠胚胎干细胞（ES）系，使delta重链基因的一个等位基因失去功能。将靶向胚胎干细胞注射到严重联合免疫缺陷小鼠的囊胚中获得嵌合小鼠，我们使用同种异型特异性试剂分析了表达靶向或野生型等位基因的胚胎干细胞衍生的B淋巴细胞。我们发现表达目标等位基因的B细胞在正常频率下以IgM+D-细胞的形式出现在外周。它们表达CD23标记物并对T细胞依赖性抗原作出反应。因此，细胞自主表达IgD既不是B细胞成熟到抗原应答状态所必需的，也不是抗原依赖性触发细胞进入免疫应答所必需的。
To analyse the function of IgD in vivo, we generated a 'loss of function' mouse model utilizing gene targeting technology. By homologous recombination in a (C57BL/6 x CBA)F1 mouse embryonic stem cell (ES) line one allele of the delta heavy chain gene was rendered non-functional. In chimeric mice obtained after injection of the targeted ES cells into blastocysts derived from severe combined immunodeficient mice we analysed ES cell derived B lymphocytes expressing the targeted or the wild-type allele by using allotype specific reagents. We show that B cells expressing the targeted allele appear in the periphery as IgM+D- cells at normal frequency. They express the CD23 marker and respond to a T cell dependent antigen. Thus, cell autonomous expression of IgD is neither essential for B cell maturation into an antigen responsive state nor for antigen dependent triggering of the cells into an immune response.