Stromal expression of 72 kda type IV collagenase (MMP-2) and TIMP-2 mRNAs in colorectal neoplasia.
Stromal expression of 72 kda type IV collagenase (MMP-2) and TIMP-2 mRNAs in colorectal neoplasia.
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发表时间：
1992-08
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通讯作者：
R. Poulsom;M. Pignatelli;M. Pignatelli;W. Stetler-Stevenson;L. Liotta;Patricia Wright;R. Jeffery;J. M. Longcroft;L. Rogers;G. Stamp;G. Stamp
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作者：
R. Poulsom;M. Pignatelli;M. Pignatelli;W. Stetler-Stevenson;L. Liotta;Patricia Wright;R. Jeffery;J. M. Longcroft;L. Rogers;G. Stamp;G. Stamp
我们进行了一项原位杂交研究，定位 12 个结直肠癌、3 个腺瘤和 4 个未受累切除边缘中 72 kda IV 型胶原酶 (MMP-2) 及其特异性抑制剂 (TIMP-2) 的 mRNA，以了解它们的分布与报告的 MMP-2 蛋白分布的相关性。在 12 个癌中的 10 个和 3 个腺瘤中的 2 个中可检测到 MMP-2 和 TIMP-2 mRNA 的标记。出乎意料的是，我们发现内皮和/或（肌）成纤维细胞性质的促纤维增生基质中的间充质细胞内的 MMP-2 和 TIMP-2 mRNA 信号强得多，而不是在预期 MMP-2 定位的肿瘤上皮细胞中。我们的数据表明基质细胞可能具有合成降解基底膜的金属蛋白酶的能力，并且可能与肿瘤上皮细胞一起积极参与组织重塑和基底膜完整性的破坏，这是侵袭性肿瘤的特征。
We undertook an in situ hybridization study to localize the mRNAs for the 72 kda type IV collagenase (MMP-2) and its specific inhibitor (TIMP-2) in 12 colorectal carcinomas, 3 adenomas, and 4 uninvolved resection margins to see how their distributions correlated with that of the reported distribution of MMP-2 protein. Labeling for MMP-2 and TIMP-2 mRNAs was detectable in 10 of 12 carcinomas and in 2 of 3 adenomas. Unexpectedly, we found much stronger signals for MMP-2 and TIMP-2 mRNAs within the mesenchymal cells in the desmoplastic stroma, of endothelial and/or (myo)fibroblastic nature, rather than in tumor epithelial cells in which localization of MMP-2 was anticipated. Our data indicate that stromal cells may have the ability to synthesize a metalloproteinase that degrades basement membrane, and may together with the neoplastic epithelial cells participate actively in the tissue remodeling and disruption of the basement membrane integrity which is characteristic of invasive tumors.