Talaromyces pinophilus strain AUN-1 as a novel mycoparasite of Botrytis cinerea, the pathogen of onion scape and umbel blights
Talaromyces pinophilus strain AUN-1 as a novel mycoparasite of Botrytis cinerea, the pathogen of onion scape and umbel blights
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DOI：
10.1016/j.micres.2018.04.004
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发表时间：
2018-01-01
影响因子：
6.7
通讯作者：
Abo-Elyousr, Kamal A. M.
中科院分区：
文献类型：
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作者：
Abdel-Rahim, Ismail R.;Abo-Elyousr, Kamal A. M.
本研究旨在研究洋葱种子作物的枯萎病和伞叶枯病病原菌灰霉病菌（Botrytis cinerea）对内生真菌Talaromyces pinophilus的寄生作用。双培养试验表明，嗜松螺旋体对灰葡萄球菌的拮抗作用取决于其寄生性，形态上通过菌丝过度生长的形成来检测其寄生性。此外，接触区菌丝光镜显示嗜松线虫菌丝在灰孢杆菌菌丝的胞内渗透生长。用扫描电镜和透射电镜分析了螺旋菌丝的形成过程和穿透过程的构象。扫描电镜观察发现，嗜松螺旋体菌丝沿灰葡萄球菌菌丝生长，附着缠绕在寄主菌丝上，产生假附着胞。在穿透部位观察到宿主菌丝细胞壁明显解体。透射电镜显微照片显示，嗜松杆菌能够产生假附着胞，穿透并进入灰葡萄球菌菌丝，造成明显的细胞质破坏。研究结果表明，嗜松螺旋体的细胞壁降解酶（几丁质酶、脂肪酶和蛋白酶）在霉菌寄生过程中具有较高的活性。综上所述，本研究表明，内生的嗜pinophilus可能是一种很有前途的植物病原真菌生物防治剂，可以替代化学杀菌剂。
This study aimed to investigate the mycoparasitism of Botrytis cinerea, the pathogen of scape and umbel blights of onion seed crops, by endophytic Talaromyces pinophilus. The dual culture test showed that the antagonistic potentiality of T. pinophilus against B. cinerea depend on the mycoparasitism that was morphologically detected by the formation of mycelial overgrowth. Moreover, the light micrograph of the mycelia at the contact zone exhibited that the hyphae of T. pinophilus penetrated and grew intracellularly inside the hyphae of B. cinerea. A more illustrative figure of the establishment of coiled hyphae as well as the conformation of the penetration process was assayed by SEM and TEM analyses. SEM micrograph revealed that the hyphae of T. pinophilus grew along hyphae of B. cinerea, attached, coiled around the host hypha and generated pseudoappressorium. A clear disintegration of cell wall of the host hypha was observed at the penetration site. The micrographs of TEM exhibited the ability of T. pinophilus to produce pseudoappressorium, penetrate and then entere a hypha of B. cinerea causing distinct cytoplasmic disorganization. High activities of cell wall degrading enzymes (chitinase, lipase and protease) involved in the mycoparasitism were evaluated by the endophytic T. pinophilus. In conclusion, this study demonstrated that the endophytic T. pinophilus may be a promising biocontrol agent against phytopathogenic fungi instead of chemical fungicides.