Localization of human pituitary hormones by multiple immunoenzyme staining procedures using monoclonal and polyclonal antibodies.
Localization of human pituitary hormones by multiple immunoenzyme staining procedures using monoclonal and polyclonal antibodies.
复制标题
使用单克隆和多克隆抗体通过多种免疫酶染色程序定位人类垂体激素。
DOI：
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发表时间：
1986
影响因子：
3.2
通讯作者：
J. Polak
中科院分区：
文献类型：
--
作者：
S. Van Noorden;M. Stuart;A. Cheung;E. Adams;J. Polak
Mouse monoclonal and rabbit polyclonal antibodies to human pituitary hormones were applied together to sections of normal and neoplastic human pituitary tissue. Binding sites were revealed with species-specific immune reagents combined with various enzymes (peroxidase, alkaline phosphatase, and beta-D-galactosidase). The enzymes were developed separately to give differently colored end-products. Where two hormones were present in the same cell, a mixed color was produced. Up to four hormones could be immunostained in a single section. Multiple immunoenzymatic staining has great potential for the analysis of plural antigen production by single cells and relationships between cells producing different antigens.
