Control of daughter centriole formation by the pericentriolar material
Control of daughter centriole formation by the pericentriolar material
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DOI：
10.1038/ncb1694
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发表时间：
2008-03-01
影响因子：
21.3
通讯作者：
Khodjakov, Alexey
中科院分区：
文献类型：
--
作者：
Loncarek, Jadranka;Hergert, Polla;Khodjakov, Alexey
控制其中心粒的数量对细胞至关重要，因为多余的中心粒会导致多极有丝分裂和基因组不稳定（1，2）。正常情况下，每个成熟（母亲）中心粒上形成一个女儿中心粒（3，4）;然而，母亲中心粒可以在单个细胞周期内产生多个女儿（5，6）。防止中心粒“过度复制”的机制知之甚少。在这里，我们使用激光显微手术来检验子中心粒附着在母体细胞壁上会抑制额外子细胞形成的假设（7，8）。我们发现，物理删除的女儿诱导复制的母亲在S期停滞的细胞。当一个母亲同时形成多个女儿时，必须将所有这些女儿都切除以诱导复制。在复制过程中形成的子中心粒的数量并不总是与消融的子中心粒的数量相匹配。我们还发现，在S期阻滞的CHO细胞中，通过过表达PCM蛋白pericentrin（9）来扩大中心粒周围物质（PCM），诱导形成许多子代中心粒。我们建议，PCM云的大小与母亲中心粒限制的女儿，可以同时形成。
Controlling the number of its centrioles is vital for the cell, as supernumerary centrioles cause multipolar mitosis and genomic instability(1,2). Normally, one daughter centriole forms on each mature (mother) centriole(3,4); however, a mother centriole can produce multiple daughters within a single cell cycle(5,6). The mechanisms that prevent centriole 'overduplication' are poorly understood. Here we use laser microsurgery to test the hypothesis that attachment of the daughter centriole to the wall of the mother inhibits formation of additional daughters(7,8). We show that physical removal of the daughter induces reduplication of the mother in S-phase-arrested cells. Under conditions when multiple daughters form simultaneously on a single mother, all of these daughters must be removed to induce reduplication. The number of daughter centrioles that form during reduplication does not always match the number of ablated daughter centrioles. We also find that exaggeration of the pericentriolar material (PCM) by overexpression of the PCM protein pericentrin(9) in S-phase-arrested CHO cells induces formation of numerous daughter centrioles. We propose that that the size of the PCM cloud associated with the mother centriole restricts the number of daughters that can form simultaneously.