Circulating microRNA-214 and -126 as potential biomarkers for canine neoplastic disease.
Circulating microRNA-214 and -126 as potential biomarkers for canine neoplastic disease.
复制标题
DOI：
10.1038/s41598-017-02607-1
复制
发表时间：
2017-05-23
影响因子：
4.6
通讯作者：
Mori T
中科院分区：
文献类型：
--
作者：
Heishima K;Ichikawa Y;Yoshida K;Iwasaki R;Sakai H;Nakagawa T;Tanaka Y;Hoshino Y;Okamura Y;Murakami M;Maruo K;Akao Y;Mori T
血液中的循环microRNA可以提供关于犬肿瘤疾病的诊断和预后信息，并且它们的谱在人类和犬种之间可能是保守的。我们采用RT-qPCR方法检测了181例肿瘤性疾病犬和健康犬的循环血浆microRNA-214和-126水平。microRNA-214水平在2种上皮性肿瘤（甲状腺癌和乳腺癌）和4种非上皮性肿瘤（骨肉瘤、组织细胞肉瘤、软骨肉瘤和血管肉瘤）中较高。相比之下，microRNA-126水平在6种上皮性肿瘤（乳腺癌、肝细胞癌、鳞状细胞癌、甲状腺癌、移行细胞癌和腺癌）和4种非上皮性肿瘤（骨肉瘤、肥大细胞瘤、黑色素瘤和血管肉瘤）中较高。microRNA-214在肉瘤中的诊断潜力相对较高，而microR-126在大多数类型的肿瘤中的诊断潜力较高。MicroRNA-214和-126分别是2组（腺癌和非上皮肿瘤，骨肉瘤除外）和3组（上皮肿瘤、腺癌和黑色素瘤）的预后预测因子。此外，microRNA水平与其他临床参数没有显示出强相关性。总之，循环microRNA-214和-126有可能成为犬肿瘤性疾病的诊断和预后生物标志物。此外，它们的特征也可能是探索人类癌症新生物标志物的关键参考。
Circulating microRNAs in the blood may provide diagnostic and prognostic information about canine neoplastic diseases, and their profiles may be conserved between human and canine species. We performed RT-qPCR to obtain the profiles of circulating plasma microRNA-214 and -126 in total 181 cases of canine neoplastic diseases and healthy controls. MicroRNA-214 levels were high in 2 epithelial tumours (thyroid and mammary carcinomas) and 4 non-epithelial tumours (osteosarcoma, histiocytic sarcoma, chondrosarcoma, and hemangiosarcoma). In contrast, microRNA-126 levels were high in 6 epithelial tumours (mammary, hepatocellular, squamous cell, thyroid, transitional cell carcinomas, and adenocarcinoma) and 4 non-epithelial tumours (osteosarcoma, mast cell tumour, melanoma, and hemangiosarcoma). The diagnostic potential of microRNA-214 was relatively high in sarcomas, whereas that of microR-126 was high in most types of the tumours. MicroRNA-214 and -126 were prognostic predictors in 2 groups (adenocarcinoma and non-epithelial tumours except for osteosarcoma) and 3 groups (epithelial tumours, adenocarcinoma, and melanoma), respectively. Additionally, the microRNA levels did not show a strong correlation with the other clinical parameters. In conclusion, circulating microRNA-214 and -126 have the potential to be diagnostic and prognostic biomarkers for canine neoplastic diseases. Furthermore, their profiles may be key references as well for exploring novel biomarkers for human cancers.