Evolution of the protease-activated receptor family in vertebrates.
Evolution of the protease-activated receptor family in vertebrates.
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脊椎动物蛋白酶激活受体家族的进化。
DOI：
10.3892/ijmm.2016.2464
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发表时间：
2016-03
影响因子：
5.4
通讯作者：
Wei JF
中科院分区：
文献类型：
--
作者：
Jin M;Yang HW;Tao AL;Wei JF
蛋白酶激活受体(PARs)属于G蛋白偶联受体家族，由4个成员组成，即PAR1-4。PARs通过凝血酶、丝氨酸和其他蛋白酶介导细胞的激活。这种由蛋白酶触发的信号事件被认为是止血、血栓形成和其他正常病理过程的关键。在本研究中，我们基于57个脊椎动物基因序列中的169个功能基因序列，通过分析系统发育树、染色体位置、选择压力和功能分歧来研究PARS的进化。通过系统发育树分析，我们发现这4个PARs起源于4个无脊椎动物祖先。选择压力结果表明，在进化过程中，只有PAR1通过正向选择出现，而其他PAR成员没有。此外，我们注意到，虽然这些PARs是独立进化的，但功能分歧的结果表明，它们的进化率相似，功能没有明显差异。我们的研究结果为了解脊椎动物PAR家族的进化史提供了有价值的见解。
Belonging to the G protein-coupled receptor (GPcr) family, the protease-activated receptors (Pars) consist of 4 members, PAR1-4. PARs mediate the activation of cells via thrombin, serine and other proteases. Such protease-triggered signaling events are thought to be critical for hemostasis, thrombosis and other normal pathological processes. In the present study, we examined the evolution of PARs by analyzing phylogenetic trees, chromosome location, selective pressure and functional divergence based on the 169 functional gene alignment sequences from 57 vertebrate gene sequences. We found that the 4 PARs originated from 4 invertebrate ancestors by phylogenetic trees analysis. The selective pressure results revealed that only PAR1 appeared by positive selection during its evolution, while the other PAR members did not. In addition, we noticed that although these PARs evolved separately, the results of functional divergence indicated that their evolutional rates were similar and their functions did not significantly diverge. The findings of our study provide valuable insight into the evolutionary history of the vertebrate PAR family.