Change of karyoskeleton during mammalian spermatogenesis: Expression pattern of nuclear lamin C2 and its regulation
Change of karyoskeleton during mammalian spermatogenesis: Expression pattern of nuclear lamin C2 and its regulation
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DOI：
10.1006/excr.1996.0315
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发表时间：
1996-11-01
影响因子：
3.7
通讯作者：
Benavente, R
中科院分区：
文献类型：
--
作者：
Alsheimer, M;Benavente, R
核纤层蛋白是一个多基因家族的主要核骨架蛋白。核纤层蛋白家族成员的表达模式已被证明是发育调节的。特别令人感兴趣的发现是，哺乳动物生精细胞表达两种小核纤层蛋白亚型（B3和C2），它们是其体细胞对应物（分别为核纤层蛋白B2和C）的较短剪接变体。虽然核纤层蛋白B3和C2似乎是特定的生殖系，这些蛋白质的表达模式和定位是未知的。在本研究中，我们提供的证据表明，在大鼠精子发生过程中，核纤层蛋白C2选择性地表达于精母细胞，即，正在进行减数分裂的细胞。与该家族其他成员一样，核纤层蛋白C2在核周围被检测到。北方印迹和原位杂交表明核纤层蛋白C2在减数分裂中的表达在转录水平上受到调控。这与两栖动物卵子发生过程中的情况相反，其中核纤层蛋白的表达在很大程度上是转录后调节的。有趣的是，核纤层蛋白钙的表达模式在时间上与另一个减数分裂特异性核骨架结构的结构蛋白组分，联会复合体（SC）相一致。两者合计，我们得出结论，配对和重组的同源染色体在减数分裂前期是伴随着显着的变化，这是由阶段特异性蛋白质的表达完成的组织的核骨架。(C)出版社：Academic Press，Inc.
Nuclear lamins are a multigene family of major karyoskeletal proteins. The expression pattern of members of the lamin family has been shown to be developmentally regulated. Of particular interest have been the findings that mammalian spermatogenic cells express two small lamin isoforms (B3 and C2) which are shorter splicing variants of their somatic counterparts (the lamins B2 and C, respectively). Although lamins B3 and C2 appear to be specific for the germ line, the expression pattern and localization of these proteins is not known. In the present study, we present evidence that during rat spermatogenesis lamin C2 is selectively expressed in spermatocytes, i.e., the cells undergoing meiosis. As in the case of other members of the family, lamin C2 was detected at the nuclear periphery. Northern blotting and in situ hybridization indicate that meiotic expression of lamin C2 is regulated at the transcriptional level. This is in contrast to the situation during amphibian oogenesis, where lamin expression is largely posttranscriptionally regulated. Interestingly, the expression pattern of lamin Ca temporally coincides with that of structural protein components of another meiosis-specific karyoskeletal structure, the synaptonemal complex (SC). Taken together, we conclude that pairing and recombination of homologous chromosomes during meiotic prophase is accompanied by significant changes in the organization of the karyoskeleton which are accomplished by the expression of stage-specific proteins. (C) 1996 Academic Press, Inc.