Expression of Genes Involved in the Non-Neuronal Cholinergic System and Their Possible Functions during Ovarian Follicular Development in Mice
Expression of Genes Involved in the Non-Neuronal Cholinergic System and Their Possible Functions during Ovarian Follicular Development in Mice
复制标题
小鼠卵泡发育过程中非神经胆碱能系统相关基因的表达及其可能的功能
DOI：
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
K. Sugiura
中科院分区：
文献类型：
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作者：
Mizuki Ueno;Haruka Ito;Takuya Kanke;Yuta Matsuno;W. Fujii;K. Naito;K. Sugiura
摘要：非神经元胆碱能系统的调节作用，如其在卵泡发育过程中对壁颗粒细胞的生长促进作用，已在几种哺乳动物中报道;然而，其在卵泡早期阶段的作用还不清楚。本研究旨在探讨小鼠卵巢非神经元胆碱能系统相关转录物的表达，以及激活乙酰胆碱（ACh）信号的影响。在卵巢和分离的壁颗粒细胞中检测到ACh代谢和烟碱胆碱能受体CHRNA 7的关键过程中所需的编码蛋白质的转录本。胆碱能激动剂卡巴胆碱刺激，促进体外壁颗粒细胞的增殖。此外，卡巴胆碱治疗的新生儿卵巢显着增加原始卵泡的数量相比，控制未处理的卵巢在器官培养。这些结果表明，一个功能性的非神经元胆碱能系统存在于小鼠卵巢，它促进壁颗粒细胞的增殖和原始卵泡的形成/存活。
Abstract: The regulatory roles of the non-neuronal cholinergic system, such as its growth promoting effects on mural granulosa cells during ovarian folliculogenesis, have been reported for several mammalian species; however, its roles in the early stages of follicles are not well-understood. This study was conducted to examine the expression of transcripts involved in the non-neuronal cholinergic system, and the effects of activating acetylcholine (ACh) signaling in mouse ovaries. The transcripts encoding proteins required in processes critical for ACh metabolism and a nicotinic cholinergic receptor, CHRNA7, were detected in ovaries and isolated mural granulosa cells. Stimulation with carbachol, a cholinergic agonist, promoted proliferation of mural granulosa cells in vitro. Moreover, carbachol treatment of neonatal ovaries significantly increased the numbers of primordial follicles compared with control untreated ovaries in organ cultures. These results suggest that a functional non-neuronal cholinergic system exists in mouse ovaries, and that it promotes the proliferation of mural granulosa cells and the formation/survival of primordial follicles.
灵长类卵巢中毒蕈碱受体类型的表达和非神经元乙酰胆碱合成的证据。
DOI：
10.1210/jcem.86.1.7146
发表时间：
2001
期刊：
The Journal of clinical endocrinology and metabolism.
影响因子：
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作者：
Fritz,S;Wessler,I;Breitling,R;Rossmanith,W;Ojeda,SR;Dissen,GA;Amsterdam,A;Mayerhofer,A
通讯作者：
Mayerhofer,A
影响因子：
4.8
作者：
Emori, Chihiro;Wigglesworth, Karen;Sugiura, Koji
通讯作者：
Sugiura, Koji
影响因子：
9
作者：
通讯作者：
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