Expression of the c-kit protein is associated with certain subtypes of salivary gland carcinoma
Expression of the c-kit protein is associated with certain subtypes of salivary gland carcinoma
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DOI：
10.1016/s0304-3835(00)00387-6
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发表时间：
2000-06-01
期刊：
影响因子：
9.7
通讯作者：
Hsu, HC
中科院分区：
文献类型：
--
作者：
Jeng, YM;Lin, CY;Hsu, HC
c-Kit 蛋白是一种受体型酪氨酸激酶，在造血细胞、黑素细胞和生殖细胞的发育中发挥重要作用，在肥大细胞增多症、胃肠道间质细胞肿瘤 (GIST)、生殖细胞肿瘤和其他几种肿瘤中表达。外显子 11 和外显子 17 的功能获得性突变已被证明是某些肿瘤中 c-kit 激活的机制。为了研究 c-kit 在唾液腺癌中的作用，我们通过免疫组织化学分析了 79 例大唾液腺癌和小唾液腺癌中 c-kit 蛋白的表达。尽管染色强度不同，但 c-kit 表达在腺样囊性癌 (20/25)、淋巴上皮瘤样癌 (6/6) 和肌上皮癌 (2/2) 中非常常见，但在其他类型的唾液腺癌 (0/46) 中不表达，P < 0.00001。通过DNA测序，在表达c-kit蛋白的所有三种唾液腺癌中均未发现c-kit近膜结构域（外显子11）和酪氨酸激酶结构域（外显子17）的遗传改变。总之，c-kit蛋白过度表达参与了某些类型唾液腺癌的发病机制，但基因突变并不是c-kit激活的机制。 (C) 2000 Elsevier Science Ireland Ltd. 保留所有权利。
The c-Kit protein, a receptor type tyrosine kinase that plays an important role in the development of hematopoietic cells, melanocytes, and germ cells, is expressed in mastocytosis, gastrointestinal stromal cell turners (GISTs), germ cell tumors, and several other tumors. Gain-of-function mutations in exon 11 and exon 17 have been shown as a mechanism of c-kit activation in some tumors. To study the role of c-kit in salivary gland carcinomas, we analyzed the c-kit protein expression in 79 carcinomas of major and minor salivary glands by immunohistochemistry. Although varying in intensity of staining, c-kit expression was identified very often in adenoid cystic carcinomas (20/25), lymphoepithelioma-like carcinomas (6/6) and myoepithelial carcinomas (2/2), but not in other types of salivary gland carcinoma (0/46), P < 0.00001. By DNA sequencing, genetic alteration of c-kit juxtamembrane domain (exon 11) and tyrosine kinase domain (exon 17) was not found in all the three types of salivary carcinoma that had c-kit protein expression. In conclusion, c-kit protein overexpression is involved in the pathogenesis of certain types of salivary gland carcinoma, but mutation of the gene is not the mechanism of c-kit activation. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.