The endogenous antioxidant ability of royal jelly in Drosophila is independent of Keap1/Nrf2 by activating oxidoreductase activity.
The endogenous antioxidant ability of royal jelly in Drosophila is independent of Keap1/Nrf2 by activating oxidoreductase activity.
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DOI：
10.1111/1744-7917.13252
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发表时间：
2023-08
期刊：
影响因子：
4
通讯作者：
Dongjing Wen;Jiayu Xie;Yao-Xian Yuan;Lirong Shen;Yufeng Yang;Wenfeng Chen
中科院分区：
文献类型：
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作者：
Dongjing Wen;Jiayu Xie;Yao-Xian Yuan;Lirong Shen;Yufeng Yang;Wenfeng Chen
蜂王浆（RJ）是一种由工蜂下咽和下颌腺分泌的生物活性物质。人们普遍认为RJ可以减少氧化应激。然而，RJ的抗氧化活性大多是通过体外化学检测方法或通过引起氧化应激的外用药物来测定的。RJ是否能够清除细胞内源性活性氧（ROS）的产生仍然是未知的。本研究利用果蝇内源性氧化应激模型系统地研究了RJ的抗氧化特性。我们发现RJ提高了衰老果蝇的睡眠质量，但随着年龄的增长，氧化损伤增加，睡眠质量下降。在暴露于过氧化氢或神经毒性剂百草枯后，补充RJ提高了果蝇的存活率并抑制了肠道细胞中的ROS水平。此外，补充RJ可以调节内源性肠道细胞中的ROS水平，延长果蝇暴露于热应激后的寿命。睡眠不足会导致肠道细胞中ROS的积累，而RJ可以减轻睡眠不足和寿命延长引起的氧化应激的后果。从机制上讲，RJ可以防止热应激或睡眠剥夺引起的细胞氧化损伤，其体内抗氧化活性不依赖于Keap1/Nrf2信号传导。补充RJ激活了果蝇肠道中的氧化还原酶活性，表明其具有抑制内源性氧化应激和维持健康的能力，可能适用于人类。
Royal jelly (RJ) is a biologically active substance secreted by the hypopharyngeal and mandibular glands of worker honeybees. It is widely claimed that RJ reduces oxidative stress. However, the antioxidant activity of RJ has mostly been determined by in vitro chemical detection methods or by external administration drugs that cause oxidative stress. Whether RJ can clear the endogenous production of reactive oxygen species (ROS) in cells remains largely unknown. Here, we systematically investigated the antioxidant properties of RJ using several endogenous oxidative stress models of Drosophila. We found that RJ enhanced sleep quality of aging Drosophila, which is decreased due to an increase of oxidative damage with age. RJ supplementation improved survival and suppressed ROS levels in gut cells of flies upon exposure to hydrogen peroxide or to the neurotoxic agent paraquat. Moreover, RJ supplementation moderated levels of ROS in endogenous gut cells and extended lifespan after exposure of flies to heat stress. Sleep deprivation leads to accumulation of ROS in the gut cells, and RJ attenuated the consequences of oxidative stress caused by sleep loss and prolonged lifespan. Mechanistically, RJ prevented cell oxidative damage caused by heat stress or sleep deprivation, with the antioxidant activity in vivo independent of Keap1/Nrf2 signaling. RJ supplementation activated oxidoreductase activity in the guts of flies, suggesting its ability to inhibit endogenous oxidative stress and maintain health, possibly in humans.