Designing Sub-2 nm Organosilica Nanohybrids for Far-Field Super-Resolution Imaging
Designing Sub-2 nm Organosilica Nanohybrids for Far-Field Super-Resolution Imaging
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设计用于远场超分辨率成像的亚 2 nm 有机硅纳米杂化物
DOI：
10.1002/ange.201912404
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Liu Xiaogang
中科院分区：
文献类型：
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作者：
Liang Liangliang;Yan Wei;Qin Xian;Peng Xiao;Feng Han;Wang Yu;Zhu Ziyu;Liu Lingmei;Han Yu;Xu Qinghua;Qu Junle;Liu Xiaogang
受激发射耗尽(STED)显微镜可以对生物样品进行高时空分辨率的超微结构成像。介绍了一种基于荧光有机二氧化硅纳米杂化材料的STED纳米探针，其尺寸小于2 nm，量子产率接近单位。通过硅烷分子介导的缩合/脱卤化过程，缓解了重原子有机荧光团的自旋-轨道耦合(SOC)，得到了在一个杂化纳米点上具有多个发射体的明亮的荧光有机二氧化硅纳米杂化材料。当被用作STED纳米探针时，这些荧光纳米杂化材料显示出强烈的光致发光、高的生物兼容性和长期的光稳定性。利用低功率激发(0.5 μW)，延长的单态寿命和可忽略的耗尽诱导的二次激发，这些STED纳米杂化材料具有高的耗尽效率(>；96 %)，极低的饱和强度(0.54 mW，约0.188 mW cm−2)和超高的横向分辨率(约λem/28)。
Stimulated emission depletion (STED) microscopy enables ultrastructural imaging of biological samples with high spatiotemporal resolution. STED nanoprobes based on fluorescent organosilica nanohybrids featuring sub‐2 nm size and near‐unity quantum yield are presented. The spin–orbit coupling (SOC) of heavy‐atom‐rich organic fluorophores is mitigated through a silane‐molecule‐mediated condensation/dehalogenation process, resulting in bright fluorescent organosilica nanohybrids with multiple emitters in one hybrid nanodot. When harnessed as STED nanoprobes, these fluorescent nanohybrids show intense photoluminescence, high biocompatibility, and long‐term photostability. Taking advantage of the low‐power excitation (0.5 μW), prolonged singlet‐state lifetime, and negligible depletion‐induced re‐excitation, these STED nanohybrids present high depletion efficiency (>96 %), extremely low saturation intensity (0.54 mW, ca. 0.188 MW cm−2), and ultra‐high lateral resolution (ca.λem/28).