Guanine-rich sequences inhibit proofreading DNA polymerases.
Guanine-rich sequences inhibit proofreading DNA polymerases.
复制标题
富含鸟嘌呤的序列抑制校对 DNA 聚合酶
DOI：
10.1038/srep28769
复制
发表时间：
2016-06-28
影响因子：
4.6
通讯作者：
Dai ZM
中科院分区：
文献类型：
--
作者：
Zhu XJ;Sun S;Xie B;Hu X;Zhang Z;Qiu M;Dai ZM
具有校正活性的DNA聚合酶对于靶DNA的准确扩增是重要的。尽管已经做出了许多努力来改进校正DNA聚合酶，但它们比非校正DNA聚合酶更容易在PCR中失败。在这里，我们表明，校正DNA聚合酶可以被某些引物抑制。进一步的分析表明，富含G的序列，如GGGGG和GGGGGHGG可以导致PCR失败使用校对DNA聚合酶，但不是Taq DNA聚合酶。这些富含G的序列的抑制作用是由G-四链体引起的，并且是剂量依赖性的。富含G的抑制序列引物可以在较低浓度下用于PCR扩增其靶DNA片段。
DNA polymerases with proofreading activity are important for accurate amplification of target DNA. Despite numerous efforts have been made to improve the proofreading DNA polymerases, they are more susceptible to be failed in PCR than non-proofreading DNA polymerases. Here we showed that proofreading DNA polymerases can be inhibited by certain primers. Further analysis showed that G-rich sequences such as GGGGG and GGGGHGG can cause PCR failure using proofreading DNA polymerases but not Taq DNA polymerase. The inhibitory effect of these G-rich sequences is caused by G-quadruplex and is dose dependent. G-rich inhibitory sequence-containing primers can be used in PCR at a lower concentration to amplify its target DNA fragment.