Temporal Differences in Granulosa Cell Specification in the Ovary Reflect Distinct Follicle Fates in Mice
Temporal Differences in Granulosa Cell Specification in the Ovary Reflect Distinct Follicle Fates in Mice
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DOI：
10.1095/biolreprod.111.095208
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发表时间：
2012-02-01
影响因子：
3.6
通讯作者：
Capel, Blanche
中科院分区：
文献类型：
--
作者：
Mork, Lindsey;Maatouk, Danielle M.;Capel, Blanche
卵巢颗粒细胞的胚胎起源几十年来一直是一个争论的话题。通过他莫昔芬诱导的Foxl2表达细胞的谱系追踪，我们发现早期性腺中的双能支持细胞前体的后代将颗粒细胞贡献给出生后立即开始生长的卵巢髓质中的特定卵泡群体。这些前体细胞来自增殖的卵巢表面上皮，并在上调Foxl2之前进入有丝分裂停滞。成年时激活的皮质原始卵泡中的颗粒细胞来源于围产期的表面上皮，并在该阶段进入有丝分裂停滞。出生后第7天，大多数存活的卵母细胞被颗粒细胞单独包裹，从表面上皮细胞的浸润下降到无法检测的水平。这些发现增加了性别决定的标准模型的复杂性，在该模型中，成人睾丸和卵巢的支持细胞和颗粒细胞直接来源于双能性腺的支持细胞前体。
The embryonic origins of ovarian granulosa cells have been a subject of debate for decades. By tamoxifen-induced lineage tracing of Foxl2-expressing cells, we show that descendants of the bipotential supporting cell precursors in the early gonad contribute granulosa cells to a specific population of follicles in the medulla of the ovary that begin to grow immediately after birth. These precursor cells arise from the proliferative ovarian surface epithelium and enter mitotic arrest prior to upregulating Foxl2. Granulosa cells that populate the cortical primordial follicles activated in adult life derive from the surface epithelium perinatally, and enter mitotic arrest at that stage. Ingression from the surface epithelium dropped to undetectable levels by Postnatal Day 7, when most surviving oocytes were individually encapsulated by granulosa cells. These findings add complexity to the standard model of sex determination in which the Sertoli and granulosa cells of the adult testis and ovary directly stem from the supporting cell precursors of the bipotential gonad.