Lymphatic and nonlymphatic pathways of peritoneal absorption in mice: physiology versus pathology.
Lymphatic and nonlymphatic pathways of peritoneal absorption in mice: physiology versus pathology.
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小鼠腹膜吸收的淋巴和非淋巴途径：生理学与病理学。
DOI：
10.1159/000170042
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发表时间：
1992
影响因子：
3
通讯作者：
Nagy,JA
中科院分区：
文献类型：
--
作者：
Nagy,JA
结合我们对恶性腹水形成的发病机制的研究，我们分析了小鼠大分子的经腹膜转运。在这篇综述中，我总结了我们的实验结果有关的流入（运输从血液到腹腔）和流出（运输从腹腔到血液）的一些不同的示踪剂[荧光素标记的葡聚糖（FITC-D），51铬红细胞，125 I-HSA，和125 I-纤维蛋白原]。我们研究了示踪剂运输在腹水肿瘤荷瘤动物作为肿瘤生长的函数，并比较了我们的结果与正常清醒小鼠和小鼠，接受了腹膜内注射的5%牛血清白蛋白溶液，以模拟与腹水肿瘤在腹膜中的生长相关的富含蛋白质的液体积累的运输特性。我们的研究结果表明，增加流入以及受损的流出都需要启动和维持肿瘤腹水的积累。为了检验增加的流入反映增加的血管通透性的假设，我们通过荧光显微镜监测静脉注射的FITC-D示踪剂（FITC-D）进入腹膜腔的运输。为了研究外排减少的机制，我们将示踪剂外排率确定为血液中的出现率和从腹膜腔中消失的速率。我们比较了这些运输性能的可溶性以及颗粒示踪剂。我们的研究结果表明，可溶性大分子有其他途径可供使用，而颗粒示踪剂如51 Cr-RBC则不可用，并且在腹水肿瘤荷瘤动物中，淋巴途径被关闭得相当快，这是由51 Cr-RBC清除率下降来判断的。通过荧光显微镜，我们观察到腹膜内注射的可溶性大分子（FITC-D）在壁层腹膜壁的间质组织摄取，特别是在腹膜内压增加的动物中，从而证实了小鼠腹膜吸收的其他非淋巴途径。最后，我们使用微粒示踪剂51 Cr-RBC来估计腹膜淋巴引流速率，基于示踪剂从腹膜消失的速率，在正常清醒小鼠中产生1.6 µl/min的值。
In conjunction with our studies of the pathogenesis of malignant ascites formation, we have analyzed the transperitoneal transport of macromolecules in mice. In this review, I summarize our experimental results concerning the influx (transport from the blood to the peritoneal cavity) and efflux (transport from the peritoneal cavity to the blood) of a number of different tracers [fluorescein-labeled dextrans (FITC-D),51Cr-RBC,125I-HSA, and125I-fibrinogen]. We examined tracer transport in ascites tumor-bearing animals as a function of tumor growth and compared our results with transport properties obtained in normal awake mice and in mice that had received an intraperitoneal injection of a solution of 5% bovine serum albumin to simulate the protein-rich fluid accumulation associated with ascites tumor growth in the peritoneum. Our results indicate thatbothincreased influx as well as impaired efflux are required to initiate and maintain tumor ascites fluid accumulation. To test the hypothesis that increased influx reflected increased vascular permeability, we monitored transport of intravenously injected FITC-D tracers (FITC-D) into the peritoneal cavity by fluorescence microscopy. To investigate the mechanisms involved in the decreased efflux, we determined tracer efflux rates both as the rate of appearance in the blood and as the rate of disappearance from the peritoneal cavity. We compared these transport properties for both soluble as well as particulate tracers. Our results indicate that there are additional routes of egress available to soluble macromolecules not available to particulate tracers such as51Cr-RBC, and that in ascites tumor-bearing animals, the lymphatic pathway is shut off rather rapidly as judged by the decreased rate of51Cr-RBC removal. By fluorescence microscopy we observed the interstitial tissue uptake of intraperitoneally injected soluble macromolecules (FITC-D) in the parietal peritoneal wall, particularly in animals with an increased intraperitoneal pressure, thereby confirming additional nonlymphatic pathways of peritoneal absorption in mice. Finally, we used the particulate tracer51Cr-RBC to estimate the peritoneal lymphatic drainage rate, yielding a value of 1.6 µl/min in normal awake mice based on the rate of tracer disappearance from the peritoneum.