Whey and molasses as inexpensive raw materials for parallel production of biohydrogen and polyesters via a two-stage bioprocess: New routes towards a circular bioeconomy
Whey and molasses as inexpensive raw materials for parallel production of biohydrogen and polyesters via a two-stage bioprocess: New routes towards a circular bioeconomy
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DOI：
10.1016/j.jbiotec.2019.07.008
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发表时间：
2019-09-10
影响因子：
4.1
通讯作者：
Lazzeri, Andrea
中科院分区：
文献类型：
--
作者：
Carlozzi, Pietro;Touloupakis, Eleftherios;Lazzeri, Andrea
研究了连续的暗发酵和光发酵阶段，以获得有益的循环生物经济。H-2光合成与聚（3-羟基丁酸酯）（P3HB）积累是一种利用农业食品工业副产品作为生态可持续生物过程中无成本富营养介质的现代生物技术方法。乳清和糖蜜是乳清（DFEW）和糖蜜（DFEM）的暗发酵废液中非常重要的富含营养物质的副产品，乳酸菌可以将其转化为乳清和糖蜜（DFEM）的暗发酵废液。这些废水是培养紫色无硫细菌的合适培养基，紫色无硫细菌是P3HB和H-2的有益生产者。本研究结果表明，乳酸杆菌和红假单胞菌sp. S16-VOGS3是缓解环境影响的两个代表性属。培养红假单胞菌sp. S16-VOGS3时，添加20%的DFEM时，P3HB产量最高（4.445 mg/(L.h)）；当添加30%的DFEM时，H-2产率最高，为4.46 mL/(L.h)。
Consecutive dark-fermentation and photo-fermentation stages were investigated for a profitable circular bioeconomy. H-2 photo-production versus poly(3-hydroxybutyrate) (P3HB) accumulation is a modern biotechnological approach to use agro-food industrial byproducts as no-cost rich-nutrient medium in eco-sustainable biological processes. Whey and molasses are very important byproducts rich in nutrients that lactic acid bacteria can convert, by dark-fermentation, in dark fermented effluents of whey (DFEW) and molasses (DFEM). These effluents are proper media for culturing purple non-sulfur bacteria, which are profitable producers of P3HB and H-2. The results of the present study show that Lactobacillus sp. and Rhodopseudomonas sp. S16-VOGS3 are two representative genera for mitigation of environmental impact. The highest productivity of P3HB (4.445 mg/(L.h)) was achieved culturing Rhodopseudomonas sp. S16-VOGS3, when feeding the bacterium with 20% of DFEM; the highest H-2 production rate of 4.46 mL/(L.h) was achieved when feeding the bacterium with 30% of DFEM.