ANTIGENIC VARIATION IN THE HEMAGGLUTININ-NEURAMINIDASE PROTEIN OF HUMAN PARA-INFLUENZA TYPE-3 VIRUS
ANTIGENIC VARIATION IN THE HEMAGGLUTININ-NEURAMINIDASE PROTEIN OF HUMAN PARA-INFLUENZA TYPE-3 VIRUS
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DOI：
10.1016/0042-6822(85)90395-2
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发表时间：
1985-01-01
期刊：
影响因子：
3.7
通讯作者：
MURPHY, BR
中科院分区：
文献类型：
--
作者：
COELINGH, KLV;WINTER, C;MURPHY, BR
生产了 16 种针对 1957 年分离的 3 型副流感病毒 (PIV3) 血凝素神经氨酸酶 (HN) 蛋白的单克隆抗体，并用于检查临床毒株的抗原变异。在存在单克隆抗体的情况下对体外选择的抗原变体的血凝抑制反应模式的分析表明，在HN分子上可检测到至少六个不同的表位。竞争性结合测定表明这些表位位于两个拓扑上不重叠的抗原位点。当测试 26 年来在三个地理区域分离的 37 个 PIV3 临床毒株与抗体的反应性时，又检测到了 4 个表位。在我们的单克隆抗体定义的 10 个独特表位中，在检查的 37 个菌株中，有 5 个没有发生可检测到的抗原变异。这些结果是预料之中的，因为 PIV3 病毒已被定性为抗原单型。相反，在其余五个表位中检测到抗原变异。这种变异的特征并不在于抗原改变随时间的累积（如甲型流感病毒），但似乎代表了 PIV3 群体内的遗传异质性。
Sixteen monoclonal antibodies directed to the hemagglutinin-neuraminidase (HN) protein of a 1957 isolate of parainfluenza type 3 virus (PIV3) were produced and used to examine antigenic variation in clinical strains. Analysis of hemagglutination-inhibition reactivity patterns of antigenic variants selectedin vitroin the presence of monoclonal antibodies indicated that there were a minimum of six distinct epitopes detectable on the HN molecule. Competitive-binding assays indicated that these epitopes were located in two topologically nonoverlapping antigenic sites. An additional four epitopes were detected when 37 PIV3 clinical strains isolated over a period of 26 years in three geographic regions were tested for reactivity with the antibodies. Of the 10 unique epitopes defined by our monoclonal antibodies, 5 did not undergo detectable antigenic variation in any of the 37 strains examined. These results were expected since PIV3 viruses have been characterized as being antigenically monotypic. In contrast, antigenic variation was detected in the remaining five epitopes. This variation was not characterized by the accumulation of antigenic alterations with time (as for influenza A viruses), but appeared to represent genetic heterogeneity within the PIV3 population.